{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:17:56Z","timestamp":1777940276096,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The accurate diagnosis of thyroid tumors is challenging. Proteomics has emerged as a promising approach for the discovery of molecular diagnostic markers as a potential complement to routine diagnostics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Protein fractions from 29 frozen thyroid tumor tissue samples (10 papillary carcinomas, 9 follicular carcinomas, and 10 follicular adenomas) as well as from normal thyroid tissue were analyzed by surface enhanced laser desorption\/ionization time-of-flight mass spectrometry followed by validation by Western blotting and immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A Ca\n                      <jats:sup>2+<\/jats:sup>\n                      -binding protein belonging to the S100 family, S100A6, was differentially expressed between papillary and follicular thyroid tumors. Moreover, two posttranslationally modified forms of S100A6 were observed and verified by liquid chromatography-coupled tandem mass spectrometry. Validation by Western blotting displayed a significantly higher expression of S100A6 in papillary thyroid carcinoma (PTC) in comparison with the other tumor groups or normal tissue (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Immunohistochemical analysis on 98 tumors showed that PTC cases had a significantly stronger cytosolic staining and a larger proportion of stained nuclei than follicular tumors.\n                      <jats:italic toggle=\"yes\">BRAF<\/jats:italic>\n                      gene mutation was not significantly associated with S100A6 protein levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>This study supports a role of S100A6 in thyroid tumorigenesis and as a potential aid in the discrimination between follicular thyroid tumors and PTC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2009.0400","type":"journal-article","created":{"date-parts":[[2010,7,14]],"date-time":"2010-07-14T18:50:12Z","timestamp":1279133412000},"page":"1067-1076","source":"Crossref","is-referenced-by-count":33,"title":["Proteomic Study of Thyroid Tumors Reveals Frequent Up-Regulation of the Ca\n                    <sup>2+<\/sup>\n                    -Binding Protein S100A6 in Papillary Thyroid Carcinoma"],"prefix":"10.1177","volume":"20","author":[{"given":"Anastasios","family":"Sofiadis","sequence":"first","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]},{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrii","family":"Dinets","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]},{"name":"Karolinska University Hospital","place":["Sweden"]},{"name":"Kyiv City Teaching Endocrinological Center","place":["Ukraine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas M.","family":"Orre","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui M.","family":"Branca","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl Christofer","family":"Juhlin","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]},{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodoros","family":"Foukakis","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]},{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00f6ran","family":"Wallin","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"H\u00f6\u00f6g","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]},{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mykola","family":"Hulchiy","sequence":"additional","affiliation":[{"name":"Kyiv City Teaching Endocrinological Center","place":["Ukraine"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Zedenius","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catharina","family":"Larsson","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janne","family":"Lehti\u00f6","sequence":"additional","affiliation":[{"name":"Karolinska University Hospital","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,10,1]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"49","volume-title":"WHO Classification of Tumours: Pathology and Genetics of Tumours of Endocrine Organs","author":"DeLellis RA","year":"2004","unstructured":"DeLellis RA, , Lloyd RV, , Heitz PU, , Eng C. 2004. 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