{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:39:50Z","timestamp":1791272390443,"version":"4.1.0"},"reference-count":49,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to compare the detection of\n                      <jats:italic toggle=\"yes\">\n                        BRAF\n                        <jats:sup>V600E<\/jats:sup>\n                      <\/jats:italic>\n                      by immunohistochemistry (IHC) using a mutation-specific antibody with molecular biology methods for evaluation of papillary thyroid carcinoma (PTC) patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and methods:<\/jats:title>\n                    <jats:p>\n                      This study concerned 198 consecutive conventional PTC patients, of which the majority were women (133\/198; 67%), with a mean age of 56 years (range 19\u201379 years).\n                      <jats:italic toggle=\"yes\">BRAF<\/jats:italic>\n                      mutation analysis was performed using DNA-based (direct sequencing, pyrosequencing, and SNaPshot) and IHC (VE1 antibody) methods. The sensitivity and specificity of IHC for BRAF\n                      <jats:sup>V600E<\/jats:sup>\n                      was compared with the molecular biology data.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A\n                      <jats:italic toggle=\"yes\">BRAF<\/jats:italic>\n                      mutational result was obtained in 194 cases. A\n                      <jats:italic toggle=\"yes\">\n                        BRAF\n                        <jats:sup>V600E<\/jats:sup>\n                      <\/jats:italic>\n                      mutation was detected in 153\/194 (79%) cases of PTC when using at least one molecular method, and in 151\/194 (78%) cases with IHC. No false positive results were noted using IHC to detect the\n                      <jats:italic toggle=\"yes\">\n                        BRAF\n                        <jats:sup>V600E<\/jats:sup>\n                      <\/jats:italic>\n                      mutation. Besides this mutation, other rare\n                      <jats:italic toggle=\"yes\">BRAF<\/jats:italic>\n                      mutations (\n                      <jats:italic toggle=\"yes\">\n                        BRAF\n                        <jats:sup>V600K<\/jats:sup>\n                      <\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">\n                        BRAF\n                        <jats:sup>K601E<\/jats:sup>\n                      <\/jats:italic>\n                      ), used as negative controls, were consistently negative with IHC. The sensitivity and specificity of IHC for the detection of this mutation were 98.7% and 100% respectively. The IHC test demonstrated excellent performance at a level equivalent to two DNA-based counterparts (pyrosequencing and SNaPshot). Failure to achieve a result was more frequent with the direct sequencing method than with the three other methods.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      IHC using the VE1 antibody is a specific and sensitive method for the detection of the\n                      <jats:italic toggle=\"yes\">\n                        BRAF\n                        <jats:sup>V600E<\/jats:sup>\n                      <\/jats:italic>\n                      mutation in PTC. IHC may be an alternative to molecular biology approaches for the routine detection of this mutation in PTC patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2013.0302","type":"journal-article","created":{"date-parts":[[2014,1,13]],"date-time":"2014-01-13T14:40:24Z","timestamp":1389624024000},"page":"858-866","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":54,"title":["Diagnostic Value of Immunohistochemistry for the Detection of the\n                    <i>\n                      BRAF\n                      <sup>V600E<\/sup>\n                    <\/i>\n                    Mutation in Papillary Thyroid Carcinoma: Comparative Analysis with Three DNA-Based Assays"],"prefix":"10.1177","volume":"24","author":[{"given":"Marius I.","family":"Ilie","sequence":"first","affiliation":[{"name":"Team 3, Faculty of Medicine, French Institute of Health and Medical Research (INSERM) 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