{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T12:33:11Z","timestamp":1784637191426,"version":"3.55.0"},"reference-count":52,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Studies of thyroid stem\/progenitor cells have been hampered due to the small organ size and lack of tissue, which limits the yield of these cells. A continuous source that allows the study and characterization of thyroid stem\/progenitor cells is desired to push the field forward.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>\n                      A cell line was established from Hoechst-resistant side population cells derived from mouse thyroid that were previously shown to contain stem\/progenitor-like cells. Characterization of these cells were carried out by using\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      two- and three-dimensional cultures and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      reconstitution of mice after orthotopic or intravenous injection, in conjunction with quantitative reverse transcription polymerase chain reaction, Western blotting, immunohisto(cyto)chemistry\/immunofluorescence, and RNA seq analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      These cells were named SPTL (\n                      <jats:underline>s<\/jats:underline>\n                      ide\n                      <jats:underline>p<\/jats:underline>\n                      opulation cell-derived\n                      <jats:underline>t<\/jats:underline>\n                      hyroid cell\n                      <jats:underline>l<\/jats:underline>\n                      ine). Under low serum culturing conditions, SPTL cells expressed the thyroid differentiation marker NKX2-1, a transcription factor critical for thyroid differentiation and function, while no expression of other thyroid differentiation marker genes were observed. SPTL cells formed follicle-like structures in Matrigel\n                      <jats:sup>\u00ae<\/jats:sup>\n                      cultures, which did not express thyroid differentiation marker genes. In mouse models of orthotopic and intravenous injection, the latter following partial thyroidectomy, a few SPTL cells were found in part of the follicles, most of which expressed NKX2-1. SPTL cells highly express genes involved in epithelial\u2013mesenchymal transition, as demonstrated by RNA seq analysis, and exhibit a gene-expression pattern similar to anaplastic thyroid carcinoma.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>These results demonstrate that SPTL cells have the capacity to differentiate into thyroid to a limited degree. SPTL cells may provide an excellent tool to study stem cells, including cancer stem cells of the thyroid.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2016.0130","type":"journal-article","created":{"date-parts":[[2017,1,27]],"date-time":"2017-01-27T04:24:20Z","timestamp":1485491060000},"page":"460-474","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["An Adult Mouse Thyroid Side Population Cell Line that Exhibits Enriched Epithelial\u2013Mesenchymal Transition"],"prefix":"10.1177","volume":"27","author":[{"given":"Tsubasa","family":"Murata","sequence":"first","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Iwadate","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Takizawa","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Miyakoshi","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suguru","family":"Hayase","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Yang","sequence":"additional","affiliation":[{"name":"and Blood Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Cai","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigetoshi","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunio","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Leidos Biomedical Research Inc."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Wakabayashi","sequence":"additional","affiliation":[{"name":"and Blood Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhu","sequence":"additional","affiliation":[{"name":"and Blood Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shioko","family":"Kimura","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,3,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc.2014.7.2.49"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2013-0097"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/scrt381"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2013.145"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1992.72.3.667"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/019262338901700205"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/019262339702500109"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(95)00019-U"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0490"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-0281"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-07-0552"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2006.16.537"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.4.1797"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2014.0464"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1365"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2014.00055"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080801"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.1.60"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1210\/er.2003-0028"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1000598"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00435-13"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb08948.x"},{"key":"e_1_3_3_27_2","unstructured":"De Felice M Di Lauro R. 2015. 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