{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:00:08Z","timestamp":1791482408137,"version":"4.3.4"},"reference-count":65,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T00:00:00Z","timestamp":1583452800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Mutations of monocarboxylate transporter 8 (MCT8), a thyroid hormone\n                        (TH)-specific transmembrane transporter, cause a severe neurodevelopmental\n                        disorder, the Allan-Herndon-Dudley syndrome. In MCT8 deficiency, TH is not\n                        able to reach those areas of the brain where TH uptake depends on MCT8.\n                        Currently, therapeutic options for MCT8-deficient patients are missing, as\n                        TH treatment is not successful in improving neurological deficits. Available\n                        data on MCT8 protein and transcript levels indicate complex expression\n                        patterns in neural tissue depending on species, brain region, sex, and age.\n                        However, information on human MCT8 expression is still scattered and\n                        additional efforts are needed to map sites of MCT8 expression in\n                        neurovascular units and neural tissue. This is of importance because new\n                        therapeutic strategies for this disease are urgently needed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>To identify regions and time windows of MCT8 expression, we used highly\n                        specific antibodies against MCT8 to perform immunofluorescence labeling of\n                        postnatal murine brains, adult human brain tissue, and human cerebral\n                        organoids.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Qualitative and quantitative analyses of murine brain samples revealed stable\n                        levels of MCT8 protein expression in endothelial cells of the\n                            blood\n                      <jats:bold>\u2013<\/jats:bold>\n                      brain barrier (BBB), choroid plexus epithelial cells,\n                        and tanycytes during postnatal development. Conversely, the neuronal MCT8\n                        protein expression that was robustly detectable in specific brain regions of\n                        young mice strongly declined with age. Similarly, MCT8 immunoreactivity in\n                        adult human brain tissue was largely confined to endothelial cells of the\n                        BBB. Recently, cerebral organoids emerged as promising models of human\n                        neural development and our first analyses of forebrain-like organoids\n                        revealed MCT8 expression in early neuronal progenitor cell populations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>With respect to MCT8-deficient conditions, our analyses not only strongly\n                        support the contention that the BBB presents a lifelong barrier to TH uptake\n                        but also highlight the need to decipher the TH transport role of MCT8 in\n                        early neuronal cell populations in more detail. Improving the understanding\n                        of the spatiotemporal expression in latter barriers will be critical for\n                        therapeutic strategies addressing MCT8 deficiency in the future.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2019.0544","type":"journal-article","created":{"date-parts":[[2020,3,7]],"date-time":"2020-03-07T02:43:10Z","timestamp":1583548990000},"page":"1366-1383","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":43,"title":["Spatiotemporal Changes of Cerebral Monocarboxylate Transporter 8                    Expression"],"prefix":"10.1177","volume":"30","author":[{"given":"Nina-Maria","family":"Wilpert","sequence":"first","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                    Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of\r                    Health, Institute of Experimental Pediatric Endocrinology, Berlin,\r                    Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Krueger","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of Leipzig, Leipzig,\r                    Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Opitz","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                    Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of\r                    Health, Institute of Experimental Pediatric Endocrinology, Berlin,\r                    Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sebinger","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                  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Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Schulz","sequence":"additional","affiliation":[{"name":"Rudolf Sch\u00f6nheimer Institute of Biochemistry, Molecular\r                    Biochemistry, University of Leipzig, Leipzig, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Spranger","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                    Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of\r                    Health, Department of Endocrinology, Diabetes and Nutrition, Berlin,\r                    Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva K.","family":"Wirth","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                    Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of\r                    Health, Department of Endocrinology, Diabetes and Nutrition, Berlin,\r                    Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Stachelscheid","sequence":"additional","affiliation":[{"name":"Stem Cell Core Facility, Berlin Institute of Health, Berlin,\r                    Germany."},{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Berlin-Brandenburg Center\r                    for Regenerative Therapies (BCRT), Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Mergenthaler","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                    Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of\r                    Health, Department of Experimental Neurology, Department of Neurology, Center\r                    for Stroke Research Berlin, Berlin, Germany."},{"name":"Berlin Institute of Health (BIH), Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vajkoczy","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                    Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of\r                    Health, Department of Neurosurgery, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heiko","family":"Krude","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, corporate member of Freie\r                    Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of\r                    Health, Institute of Experimental Pediatric Endocrinology, Berlin,\r                    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