{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:56:50Z","timestamp":1790488610444,"version":"4.1.0"},"reference-count":59,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Development of adipose tissue before birth is essential for energy storage and thermoregulation in the neonate and for cardiometabolic health in later life. Thyroid hormones are important regulators of growth and maturation in fetal tissues. Offspring hypothyroid\n                      <jats:italic toggle=\"yes\">in utero<\/jats:italic>\n                      are poorly adapted to regulate body temperature at birth and are at risk of becoming obese and insulin resistant in childhood. The mechanisms by which thyroid hormones regulate the growth and development of adipose tissue in the fetus, however, are unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>This study examined the structure, transcriptome, and protein expression of perirenal adipose tissue (PAT) in a fetal sheep model of thyroid hormone deficiency during late gestation. Proportions of unilocular (UL) (white) and multilocular (ML) (brown) adipocytes, and UL adipocyte size, were assessed by histological and stereological techniques. Changes to the adipose transcriptome were investigated by RNA sequencing and bioinformatic analysis, and proteins of interest were quantified by Western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Hypothyroidism\n                      <jats:italic toggle=\"yes\">in utero<\/jats:italic>\n                      resulted in elevated plasma insulin and leptin concentrations and overgrowth of PAT in the fetus, specifically due to hyperplasia and hypertrophy of UL adipocytes with no change in ML adipocyte mass. RNA sequencing and genomic analyses showed that thyroid deficiency affected 34% of the genes identified in fetal adipose tissue. Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathways were associated with adipogenic, metabolic, and thermoregulatory processes, insulin resistance, and a range of endocrine and adipocytokine signaling pathways. Adipose protein levels of signaling molecules, including phosphorylated S6-kinase (pS6K), glucose transporter isoform 4 (GLUT4), and peroxisome proliferator-activated receptor \u03b3 (PPAR\u03b3), were increased by fetal hypothyroidism. Fetal thyroid deficiency decreased uncoupling protein 1 (UCP1) protein and mRNA content, and UCP1 thermogenic capacity without any change in ML adipocyte mass.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      Growth and development of adipose tissue before birth is sensitive to thyroid hormone status\n                      <jats:italic toggle=\"yes\">in utero<\/jats:italic>\n                      . Changes to the adipose transcriptome and phenotype observed in the hypothyroid fetus may have consequences for neonatal survival and the risk of obesity and metabolic dysfunction in later life.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2019.0749","type":"journal-article","created":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T04:50:21Z","timestamp":1582087821000},"page":"794-805","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["Thyroid Deficiency Before Birth Alters the Adipose Transcriptome to Promote Overgrowth of White Adipose Tissue and Impair Thermogenic Capacity"],"prefix":"10.1177","volume":"30","author":[{"given":"Shelley E.","family":"Harris","sequence":"first","affiliation":[{"name":"Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miles J.","family":"De Blasio","sequence":"additional","affiliation":[{"name":"Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Centre for Trophoblast Research, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcella","family":"Ma","sequence":"additional","affiliation":[{"name":"Genomics-Transcriptomics Core, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.B. Peter","family":"Wooding","sequence":"additional","affiliation":[{"name":"Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell S.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Centre for Trophoblast Research, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Blache","sequence":"additional","affiliation":[{"name":"School of Animal Biology, University of Western Australia, Crawley, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Meredith","sequence":"additional","affiliation":[{"name":"Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Murray","sequence":"additional","affiliation":[{"name":"Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail L.","family":"Fowden","sequence":"additional","affiliation":[{"name":"Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison J.","family":"Forhead","sequence":"additional","affiliation":[{"name":"Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom."},{"name":"Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,6,5]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2017.10.004"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2004.02116.x"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2008.00702.x"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-2315"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pedneo.2012.10.008"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/kjm2.12118"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-14-0025"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabet.2009.09.001"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12053"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-071813-105330"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1405-8"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1760023"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0310"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1071\/RD9960995"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000484386"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1055\/a-0896-0968"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00479"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/0003319718799967"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0540055"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1113\/JP273555"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1650625"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-10-0360"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1159\/000140888"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.10335.1"},{"key":"e_1_3_5_26_2","unstructured":"Andrews S Krueger F Degonds-Pichon A Biggins L Krueger C Wingett S. 2012. 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