{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:13:00Z","timestamp":1790100780980,"version":"4.0.1"},"reference-count":33,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Congenital hypothyroidism is often caused by genetic mutations that impair thyroid hormone (TH) production, resulting in growth and development defects. XB130 (actin filament associated protein 1 like 2) is an adaptor\/scaffold protein that plays important roles in cell proliferation, migration, intracellular signal transduction, and tumorigenesis. It is highly expressed in thyrocytes, however, its function in the thyroid remains largely unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice and their littermates were studied. Postnatal growth and growth hormone levels were measured, and responses to low or high-iodine diet, and levothyroxine treatment were examined. TH and thyrotropin in the serum and TH in the thyroid glands were quantified. Structure and function of thyrocytes in embryos and postnatal life were studied with histology, immunohistochemistry, immunofluorescence staining, Western blotting, and quantitative reverse transcription polymerase chain reaction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice exhibited transient growth retardation postnatally, due to congenital hypothyroidism with reduced TH synthesis and secretion, which could be rescued by exogenous thyroxine supplementation. The thyroid glands of\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice displayed diminished thyroglobulin iodination and release at both embryonic and early postnatal stages. XB130 was found mainly on the apical membrane of thyroid follicles. Thyroid glands of embryonic and postnatal\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice exhibited disorganized apical membrane structure, delayed folliculogenesis, and abnormal formation of thyroid follicle lumina.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>XB130 critically regulates folliculogenesis by maintaining apical membrane structure and function of thyrocytes, and its deficiency leads to congenital hypothyroidism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2021.0195","type":"journal-article","created":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T03:35:07Z","timestamp":1630553707000},"page":"1650-1661","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["XB130 Deficiency Causes Congenital Hypothyroidism in Mice due to Disorganized Apical Membrane Structure and Function of Thyrocytes"],"prefix":"10.1177","volume":"31","author":[{"given":"Yingchun","family":"Wang","sequence":"first","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Yan","family":"Xiang","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."},{"name":"Department of Physiology and Pharmacology, University of Western Ontario, London, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Sugihara","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Yang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, University of Western Ontario, London, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Hui","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Medicine and Chicago, Illinois, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hae-Ra","family":"Cho","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."},{"name":"Department of Physiology, University of Toronto, Toronto, Ontario, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Toba","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Hui","family":"Bai","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8418-5054","authenticated-orcid":false,"given":"Sylvia L.","family":"Asa","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Health Network, Toronto, Ontario, Canada."},{"name":"University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-8799","authenticated-orcid":false,"given":"Peter","family":"Arvan","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, Michigan, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0164-8231","authenticated-orcid":false,"given":"Samuel","family":"Refetoff","sequence":"additional","affiliation":[{"name":"Department of Medicine and Chicago, Illinois, USA."},{"name":"Department of Pediatrics; and Chicago, Illinois, USA."},{"name":"Department of Committee on Genetics; The University of Chicago, Chicago, Illinois, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9188-8417","authenticated-orcid":false,"given":"Mingyao","family":"Liu","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."},{"name":"Department of Physiology, University of Toronto, Toronto, Ontario, Canada."},{"name":"Department of Surgery, and University of Toronto, Toronto, Ontario, Canada."},{"name":"Department of Medicine; and University of Toronto, Toronto, Ontario, Canada."},{"name":"Department of Institute of Medical Science; Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,11,10]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2017.10.004"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e32834726a4"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/10507250360731550"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M806893200"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1530\/JME-12-0219"},{"key":"e_1_3_6_7_2","first-page":"1958","article-title":"Thyroid follicle development requires Smad1\/5- and endothelial cell-dependent basement membrane assembly","volume":"143","author":"Villacorte M","year":"2016","unstructured":"Villacorte M, , Delmarcelle AS, , Lernoux M, et al. 2016. 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Pax8 controls thyroid follicular polarity through cadherin-16. J Cell Sci, 130:219\u2013231.","journal-title":"J Cell Sci"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.071050"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.24.3011"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-03-0242"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.1210\/me.2011-1320"},{"key":"e_1_3_6_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/2043-9113-1-10"},{"key":"e_1_3_6_32_2","first-page":"903014","article-title":"XB130-a novel adaptor protein: gene, function, and roles in tumorigenesis","volume":"2014","author":"Bai XH","year":"2014","unstructured":"Bai XH, , Cho HR, , Moodley S, et al. 2014. XB130-a novel adaptor protein: gene, function, and roles in tumorigenesis. 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