{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:24:03Z","timestamp":1777875843976,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2021,11]]},"abstract":"\n Background:<\/jats:title>\n Epigenetic disorders play an important role in the pathogenesis of autoimmune thyroiditis (AIT). Therefore, the study of the possible role of DNA methylation in AIT is of great significance to explore the pathogenesis of AIT.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n From May 2019 to June 2019, whole blood samples were collected from 176 AIT patients and 176 controls from different water iodine levels in Shandong Province, China. We used the Illumina Methylation 850K BeadChip to determine significant differences in methylation status of genes and used the MethylTarget\u2122 assay to verify the methylation level in 176 cases and 176 controls. The relative mRNA levels of genes were detected by quantitative real-time-polymerase chain reaction.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n There were multiple differential methylation sites in the\n HLA-DPB1<\/jats:italic>\n and\n PDCD1LG2<\/jats:italic>\n genes between the case and control population with different water iodine levels. Some target regions of HLA-DPB1 and\n PDCD1LG2<\/jats:italic>\n genes were negatively correlated with relative mRNA expression in the case and control populations and with different water iodine levels.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n There is differential methylation status in genomic DNA in patients with AIT. The methylation patterns of\n HLA-DPB1<\/jats:italic>\n and\n PDCD1LG2<\/jats:italic>\n genes related to cell adhesion molecule pathway may be different based on different water iodine levels.\n HLA-DPB1<\/jats:italic>\n and\n PDCD1LG2<\/jats:italic>\n genes related to the cell adhesion molecules pathway may play a role in the development of AIT. This study is registered with Chinese Clinical Trial Registry,\n www.chictr.org.cn<\/jats:uri>\n , number ChiCTR2000039105.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/thy.2021.0221","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T05:29:43Z","timestamp":1628746183000},"page":"1741-1748","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["DNA Methylation Patterns in the\n HLA-DPB1<\/i>\n and\n PDCD1LG2<\/i>\n Gene Regions in Patients with Autoimmune Thyroiditis from Different Water Iodine Areas"],"prefix":"10.1177","volume":"31","author":[{"given":"Siyuan","family":"Wan","sequence":"first","affiliation":[{"name":"Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin City, People's Republic of China."},{"name":"Department of Preventive Medicine, Qiqihar Medical University, Qiqihar City, People's Republic of China."},{"name":"National Health Commission & Education Bureau of Heilongjiang Province, Key Laboratory of Etiology and Epidemiology, Harbin Medical University (23618504), Harbin, People's Republic of China."},{"name":"Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health, Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin City, People's Republic of China."},{"name":"National Health Commission & Education Bureau of Heilongjiang Province, Key Laboratory of Etiology and Epidemiology, Harbin Medical University (23618504), Harbin, People's Republic of China."},{"name":"Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health, Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingxuan","family":"Ren","sequence":"additional","affiliation":[{"name":"Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin City, People's Republic of China."},{"name":"National Health Commission & Education Bureau of Heilongjiang Province, Key Laboratory of Etiology and Epidemiology, Harbin Medical University (23618504), Harbin, People's Republic of China."},{"name":"Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health, Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengying","family":"Qu","sequence":"additional","affiliation":[{"name":"Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin City, People's Republic of China."},{"name":"National Health Commission & Education Bureau of Heilongjiang Province, Key Laboratory of Etiology and Epidemiology, Harbin Medical University (23618504), Harbin, People's Republic of China."},{"name":"Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health, Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaiyong","family":"Wu","sequence":"additional","affiliation":[{"name":"Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin City, People's Republic of China."},{"name":"National Health Commission & Education Bureau of Heilongjiang Province, Key Laboratory of Etiology and Epidemiology, Harbin Medical University (23618504), Harbin, People's Republic of China."},{"name":"Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health, Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shandong Institute of Prevention and Control for Endemic Disease, Shandong Center for Disease Control and Prevention, Jinan City, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoming","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Institute of Prevention and Control for Endemic Disease, Shandong Center for Disease Control and Prevention, Jinan City, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Shen","sequence":"additional","affiliation":[{"name":"Centre for Endemic Disease Control, Chinese Centre for Disease Control and Prevention, Harbin Medical University, Harbin City, People's Republic of China."},{"name":"National Health Commission & Education Bureau of Heilongjiang Province, Key Laboratory of Etiology and Epidemiology, Harbin Medical University (23618504), Harbin, People's Republic of China."},{"name":"Heilongjiang Provincial Key Laboratory of Trace Elements and Human Health, Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,11,10]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-0660"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2016.31.2.213"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114520001786"},{"key":"e_1_3_6_5_2","volume-title":"Eighth edition. People's Medical Publishing House (PMPH)","author":"Chen H","year":"2013","unstructured":"Chen H, , Zhong N, , Lu Z. 2013. Internal Medicine. Eighth edition. People's Medical Publishing House (PMPH), Beijing, China."},{"key":"e_1_3_6_6_2","author":"Zhang YP","unstructured":"Zhang YP, , Yan YQ, , Liu LJ, et al. 2016 Method for Determination of Iodine in Urine by As3+-Ce4+ Catalytic Spectrophotometry (WS\/T 107.1\u20132016). Health Standard of China, Beijing (In Chinese).","journal-title":"et al"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0216941"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.08.003"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.2217\/imt-2020-0037"},{"key":"e_1_3_6_10_2","first-page":"453","article-title":"Investigation on the incidence of hyperthyroidism among iodine supplied population of low-grade iodine deficiency area","volume":"18","author":"Xu W","year":"1999","unstructured":"Xu W, , Wang G, , Jin D, et al. 1999. Investigation on the incidence of hyperthyroidism among iodine supplied population of low-grade iodine deficiency area. Chin J Endemiol, 18:453\u2013455. 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(In Chinese).","journal-title":"Nat l Med J China"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145630"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014524"}],"container-title":["Thyroid\u00ae"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/thy.2021.0221","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/thy.2021.0221","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/thy.2021.0221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:08:27Z","timestamp":1777586907000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/thy.2021.0221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1089\/thy.2021.0221"],"URL":"https:\/\/doi.org\/10.1089\/thy.2021.0221","relation":{},"ISSN":["1050-7256","1557-9077"],"issn-type":[{"value":"1050-7256","type":"print"},{"value":"1557-9077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11]]}}}