{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:24:16Z","timestamp":1777875856313,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Multinodular goiter (MNG) is the most common disorder of the thyroid gland. Aging and genetic mutations that impair thyroid hormone (TH) production have been implicated in the development of MNG. XB130 is an adaptor\/scaffold protein predominantly expressed in the thyroid gland. XB130 deficiency leads to transient postnatal growth retardation in mice due to congenital hypothyroidism. We studied the formation of MNG and possible mechanisms in elderly mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Thyroid glands of male and female\n                      <jats:italic toggle=\"yes\">Xb130-<\/jats:italic>\n                      knockout (\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      ), heterozygous (\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>+\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      ), and wild-type (\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>+\/+<\/jats:sup>\n                      <\/jats:italic>\n                      ) mice at the ages of 12\u201320 months were harvested for visual examination, histopathological, and immunohistological analyses. Blood and thyroid samples were collected after feeding elderly mice with a low iodine diet for\n                      <jats:sup>125<\/jats:sup>\n                      I uptake and perchlorate discharge assay. The activity of thyroperoxidase (Tpo) was examined by spectrophotometric evaluation of iodide oxidation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      While moderate MNG was seen in\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>+\/+<\/jats:sup>\n                      <\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>+\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice, severe MNG, characterized by multiple nodules intermixed with dilated colloid-rich macrofollicles, was found only in\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice at 18 months. Thyrocyte cytoskeletal structure and cell adhesion molecules were disorganized, and TH production was significantly reduced. Reduced iodide organification was seen in elderly\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>+\/+<\/jats:sup>\n                      <\/jats:italic>\n                      mice and further enhanced in\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice. In\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>+\/+<\/jats:sup>\n                      <\/jats:italic>\n                      mice, Tpo shows high affinity with hydrogen peroxide (H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      ) throughout aging, but reduced affinity with iodide in an age-dependent manner. By contrast, in elderly\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice, the affinity of Tpo for iodide remained high, but the affinity of Tpo for H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      was reduced.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      The pathophysiological features in the thyroid glands of aged\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice closely resemble the features of MNG in humans. Moderate MNG in elderly mice was dramatically aggravated by XB130 deficiency. Reduced affinity of Tpo for H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      may contribute to MNG development via oxidative stress. This could be specific to XB130 deficiency but also could be a common mechanism in MNG. Its clinical relevance should be further investigated.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2021.0458","type":"journal-article","created":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T03:01:52Z","timestamp":1639710112000},"page":"385-396","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Pathogenesis of Multinodular Goiter in Elderly XB130-Deficient Mice: Alteration of Thyroperoxidase Affinity with Iodide and Hydrogen Peroxide"],"prefix":"10.1177","volume":"32","author":[{"given":"Hae-Ra","family":"Cho","sequence":"first","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."},{"name":"Department of Physiology, University of Toronto, Toronto, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichi","family":"Sugihara","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6463-8498","authenticated-orcid":false,"given":"Hiroki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Yan","family":"Xiang","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Bai","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingchun","family":"Wang","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hui","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Chicago, Chicago, Illinois, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8418-5054","authenticated-orcid":false,"given":"Sylvia L.","family":"Asa","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Health Network, Toronto, Canada."},{"name":"University Hospitals Cleveland Medical Center, Case Western Reserve University, Cleveland, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0164-8231","authenticated-orcid":false,"given":"Samuel","family":"Refetoff","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Chicago, Chicago, Illinois, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9188-8417","authenticated-orcid":false,"given":"Mingyao","family":"Liu","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Canada."},{"name":"Department of Physiology, University of Toronto, Toronto, Canada."},{"name":"Department of Surgery and Temerty Faculty of Medicine, University of Toronto, Toronto, Canada."},{"name":"Department of Medicine, Temerty Faculty of Medicine, University of Toronto, Toronto, Canada."},{"name":"Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, Toronto, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"key":"e_1_3_6_2_2","unstructured":"Medeires Neto G 2016 Multinodular goiter. www.endotext.org\/chapter\/multinodular-goiter. 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