{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:24:15Z","timestamp":1777875855655,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Thyroid\u00ae"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      XB130 (actin filament-associated protein 1-like 2, AFAP1L2) is a thyroid-abundant adaptor\/scaffold protein.\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice exhibit transient growth retardation postnatally due to congenital hypothyroidism with diminished thyroglobulin iodination and release at both embryonic and early postnatal stages due to disorganized thyroid apical membrane structure and function. We hypothesized that XB130 is crucial for polarity and folliculogenesis by mediating proper cytoskeletal structure and function in thyrocytes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Primary thyrocytes isolated from thyroid glands of\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice and their wild-type littermates at postnatal week 2 were cultured in 10% Matrigel for different time periods. Folliculogenesis was studied with immunofluorescence staining, followed by confocal microscopy. Cells were also transfected to express human XB130 fused Green Fluorescent Protein (XB130-GFP) or Green Fluorescent Protein (GFP) only before morphological analysis. Cytoskeletal structures from embryo and postnatal thyroid glands were also studied.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      In three-dimensional cultures of thyrocytes, XB130, aligned with actin filaments, participated in defining the site of apical membrane formation and coalescence to form a thyroid follicle lumen.\n                      <jats:italic toggle=\"yes\">\n                        Xb130\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      thyrocytes displayed delayed folliculogenesis, reduced recruitment of a microtubule (MT)-associated proteins, and disorganized acetylated tubulin under the apical membrane, resulting in delayed folliculogenesis with reduced efficiency in formation of the thyroid follicle lumen.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>XB130 critically regulates thyrocyte polarization by functioning as a link between the actin filament cortex and MT network at the apical membrane of thyrocytes. Defects of adaptor scaffold proteins may affect cellular polarity and cytoskeletal structure and function and result in disorders of epithelial function, such as congenital hypothyroidism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/thy.2021.0461","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T12:13:43Z","timestamp":1634300023000},"page":"128-137","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["XB130 Plays an Essential Role in Folliculogenesis Through Mediating Interactions Between Microfilament and Microtubule Systems in Thyrocytes"],"prefix":"10.1177","volume":"32","author":[{"given":"Yingchun","family":"Wang","sequence":"first","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Yan","family":"Xiang","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada."},{"name":"Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junichi","family":"Sugihara","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Yang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hui","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Medicine, Chicago, Illinois, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Arvan","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology &amp; Diabetes, University of Michigan, Ann Arbor, Michigan, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0164-8231","authenticated-orcid":false,"given":"Samuel","family":"Refetoff","sequence":"additional","affiliation":[{"name":"Department of Medicine, Chicago, Illinois, USA."},{"name":"Department of Pediatrics, Chicago, Illinois, USA."},{"name":"Committee on Genetics, The University of Chicago, Chicago, Illinois, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9188-8417","authenticated-orcid":false,"given":"Mingyao","family":"Liu","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada."},{"name":"Department of Surgery, Medicine and Physiology, and Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada."},{"name":"Department of Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2017.10.004"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e32834726a4"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2018-00603"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701684200"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.447"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2021.0195"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2001.020301.x"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201310021"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2016.00089"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3777"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13261"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108952"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0468"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-05-0435"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2106"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.12.003"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12426"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.194878"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218017109"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.08.009"},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1520638113"},{"key":"e_1_3_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2003.00149.x"},{"key":"e_1_3_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/421230a"},{"key":"e_1_3_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.01.094"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0169(200103)48:3<201::AID-CM1009>3.0.CO;2-C"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2010.11.024"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043646"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059057"},{"key":"e_1_3_6_30_2","first-page":"903014","article-title":"XB130\u2014a novel adaptor protein: gene, function, and roles in tumorigenesis","volume":"2014","author":"Bai XH","year":"2014","unstructured":"Bai XH, , Cho H, , Moodley S, , Liu M. 2014. 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