{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:16:44Z","timestamp":1788185804224,"version":"build-2803163510"},"reference-count":53,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>\n                    The aim of this study is to present molecular, serologic, and clinical findings for dogs that were naturally infected with\n                    <jats:italic toggle=\"yes\">Anaplasma phagocytophilum<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">Borrelia burgdorferi<\/jats:italic>\n                    sensu lato (s. l.) in the Czech Republic. This data can provide information relevant to human infection. In total, blood samples from 296 dogs and 118 engorged ticks were examined. Samples were tested for\n                    <jats:italic toggle=\"yes\">A. phagocytophilum<\/jats:italic>\n                    using polymerase chain reaction (PCR) amplification, nested PCR, and direct sequencing of the 16S rDNA, and for\n                    <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                    s. l. using PCR amplification of the 16S rDNA and restriction fragment length polymorphism analysis of the 5S-23S rDNA intergenic spacer. In addition, blood samples were screened for antibodies to these bacteria. Ten (3.4%) dogs were PCR-positive for\n                    <jats:italic toggle=\"yes\">A. phagocytophilum<\/jats:italic>\n                    . Morulae of\n                    <jats:italic toggle=\"yes\">A. phagocytophilum<\/jats:italic>\n                    in granulocytes were found in two of these dogs. Nine of the PCR-positive dogs had clinical signs related to anaplasmosis. Statistically significant differences in the PCR detection rates were found between breeds and between symptomatic and asymptomatic dogs. Infection with\n                    <jats:italic toggle=\"yes\">Borrelia garinii<\/jats:italic>\n                    was detected by PCR in a dog with meningoencephalitis. DNA of\n                    <jats:italic toggle=\"yes\">A. phagocytophilum<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                    s. l. (\n                    <jats:italic toggle=\"yes\">B. garinii<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">Borrelia afzelii<\/jats:italic>\n                    ) was detected in 8.5% and 6.8% of ticks, respectively. Immunoglobulin (Ig) G seropositivity to\n                    <jats:italic toggle=\"yes\">A. phagocytophilum<\/jats:italic>\n                    was 26%. Significant differences were found with respect to breed and gender. IgM and IgG antibodies to\n                    <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                    s. l. were detected in 2.4% and 10.3% of dogs, respectively. Our findings suggest that the exposure to\n                    <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                    s. l. exists in dogs in the Czech Republic, and exposure to\n                    <jats:italic toggle=\"yes\">A. phagocytophilum<\/jats:italic>\n                    is common.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2008.0127","type":"journal-article","created":{"date-parts":[[2009,3,9]],"date-time":"2009-03-09T11:22:32Z","timestamp":1236597752000},"page":"655-661","source":"Crossref","is-referenced-by-count":29,"title":["Detection of\n                    <i>Anaplasma phagocytophilum<\/i>\n                    and\n                    <i>Borrelia burgdorferi<\/i>\n                    Sensu Lato in Dogs in the Czech Republic"],"prefix":"10.1177","volume":"9","author":[{"given":"Katerina","family":"Kybicov\u00e1","sequence":"first","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Sch\u00e1nilec","sequence":"additional","affiliation":[{"name":"University of Veterinary and Pharmaceutical Sciences","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagmar","family":"Hul\u00ednsk\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Uherkov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Kurzov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Spejchalov\u00e1","sequence":"additional","affiliation":[{"name":"University of Veterinary and Pharmaceutical Sciences","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2009,12,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2007.0277"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.4.1005-1007.1997"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2007.0236"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1999.tb03249.x"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1987.191.11.1468"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.1104"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1992.201.07.1030"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.1.509-516.2003"},{"key":"e_1_3_3_10_1","first-page":"367","article-title":"Tick spirochetosis by Borrelia burgdorferi in dogs in the west of France. 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