{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T13:40:18Z","timestamp":1778074818537,"version":"3.51.4"},"reference-count":47,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:p>\n                    We report the construction and comparative characterization of a full-length West Nile virus (WNV) cDNA infectious clone (ic) that contains a green fluorescent protein (GFP) expression cassette fused within the viral open reading frame. Virus derived from WNV-GFP ic stably infected\n                    <jats:italic toggle=\"yes\">Culex pipiens quinquefasciatus<\/jats:italic>\n                    mosquitoes at comparable rates to virus derived from the parental (non-GFP) ic. However, insertion of this GFP cassette resulted in a temporal delay in\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    replication kinetics and significantly decreased dissemination to head tissue. Consistent with previous reports of WNV-infected mosquito midguts, focal GFP expression was observed at 3 days post-infection (dpi), with the majority of posterior midgut epithelial cells being positive by 7\u2009dpi. GFP foci were observed in one pair of salivary glands (1\/15) dissected 14\u2009dpi. Mice exposed to WNV-GFP\u2013infected mosquitoes developed viremia, and GFP was detected in lymph node homogenates. These data demonstrate the effectiveness of our strategy to generate a replication competent construct with increased reporter gene stability that may be used to study early events in infection.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2009.0067","type":"journal-article","created":{"date-parts":[[2009,7,20]],"date-time":"2009-07-20T11:53:02Z","timestamp":1248090782000},"page":"267-274","source":"Crossref","is-referenced-by-count":40,"title":["Infection, Dissemination, and Transmission of a West Nile Virus Green Fluorescent Protein Infectious Clone by\n                    <i>Culex pipiens quinquefasciatus<\/i>\n                    Mosquitoes"],"prefix":"10.1177","volume":"10","author":[{"given":"Charles E.","family":"McGee","sequence":"first","affiliation":[{"name":"University of Texas Medical Branch"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandr V.","family":"Shustov","sequence":"additional","affiliation":[{"name":"University of Texas Medical Branch"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantin","family":"Tsetsarkin","sequence":"additional","affiliation":[{"name":"University of Texas Medical Branch"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilya V.","family":"Frolov","sequence":"additional","affiliation":[{"name":"University of Texas Medical Branch"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter W.","family":"Mason","sequence":"additional","affiliation":[{"name":"University of Texas Medical Branch"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana L.","family":"Vanlandingham","sequence":"additional","affiliation":[{"name":"University of Texas Medical Branch"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Higgs","sequence":"additional","affiliation":[{"name":"University of Texas Medical Branch"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,4,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/31.2.202"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(82)90132-8"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-40.4.528"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/153036604773083013"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00316-07"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0962-1075.2004.00521.x"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.12.001"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.56.012302.160654"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/43.5.966"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2004.00464.x"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/1530366041210729"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/42.3.429"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.118"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01128-4"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-1535-0_38"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503835102"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.2144\/96214st03"},{"key":"e_1_3_3_19_1","volume-title":"International Catalogue of Arboviruses","author":"Karabatsos N","year":"1985","unstructured":"Karabatsos N. 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