{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T01:05:30Z","timestamp":1785546330187,"version":"3.56.0"},"reference-count":46,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:p>\n                    Natural sandfly infection by\n                    <jats:italic toggle=\"yes\">Leishmania<\/jats:italic>\n                    spp. in an area endemic for American cutaneous leishmaniasis was analyzed using multiplex polymerase chain reaction (PCR). The sandflies were captured using Falc\u00e3o light traps in an endemic area of the municipality of Doutor Camargo during March, April, and June 2008. In total, 1803 females were analyzed; 1755 were\n                    <jats:italic toggle=\"yes\">Nyssomyia neivai<\/jats:italic>\n                    (Pinto) and 48 were\n                    <jats:italic toggle=\"yes\">Nyssomyia whitmani<\/jats:italic>\n                    (Antunes and Coutinho). Multiplex PCR analyses using MP3H-MP1L and 5Llcac-3Llcac primers showed the presence of\n                    <jats:italic toggle=\"yes\">Leishmania<\/jats:italic>\n                    <jats:italic toggle=\"yes\">(Viannia)<\/jats:italic>\n                    spp. in 4\/181 pools of sandflies, all\n                    <jats:italic toggle=\"yes\">Ny. neivai<\/jats:italic>\n                    , that is, a minimal infection rate of 0.22%. This study showed, for the first time, the presence of DNA of\n                    <jats:italic toggle=\"yes\">Leishmania (Viannia)<\/jats:italic>\n                    spp. in\n                    <jats:italic toggle=\"yes\">Ny. neivai.<\/jats:italic>\n                    This suggests the existence of natural infection by\n                    <jats:italic toggle=\"yes\">Leishmania (Viannia)<\/jats:italic>\n                    spp. in\n                    <jats:italic toggle=\"yes\">Ny. neivai<\/jats:italic>\n                    in the state of Paran\u00e1. Multiplex PCR is an important tool in the detection of\n                    <jats:italic toggle=\"yes\">Leishmania<\/jats:italic>\n                    infection in sandflies.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2009.0218","type":"journal-article","created":{"date-parts":[[2010,12,14]],"date-time":"2010-12-14T09:32:44Z","timestamp":1292319164000},"page":"137-143","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Natural Infection of\n                    <i>Nyssomyia neivai<\/i>\n                    by\n                    <i>Leishmania (Viannia<\/i>\n                    ) spp. in the State of Paran\u00e1, Southern Brazil, Detected by Multiplex Polymerase Chain Reaction"],"prefix":"10.1177","volume":"11","author":[{"given":"Diego Molina","family":"Oliveira","sequence":"first","affiliation":[{"name":"Universidade Estadual de Maring\u00e1","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin Rosi","family":"Reinhold-Castro","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Maring\u00e1","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Vin\u00edcius Zandonadi","family":"Bernal","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Maring\u00e1","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane Machado","family":"de Oliveira Legriffon","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Maring\u00e1","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Valdrinez Campana","family":"Lonardoni","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Maring\u00e1","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ueslei","family":"Teodoro","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Maring\u00e1","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00eds Gomes Verzignassi","family":"Silveira","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Maring\u00e1","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,2,1]]},"reference":[{"key":"e_1_3_3_2_1","first-page":"207","volume-title":"Flebotom\u00edneos do Brasil","author":"Aguiar GM","year":"2003","unstructured":"Aguiar GM, , Medeiros WM. Distribui\u00e7\u00e3o regional e h\u00e1bitats das esp\u00e9cies de flebotom\u00edneos do Brasil. Rangel EF, , Lainson R. Flebotom\u00edneos do Brasil. Rio de Janeiro: editora Fiocruz, 2003; 207\u2013256."},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.5.1933-1938.2000"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2004.10.004"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2005.11.010"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0001-706X(92)90006-J"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(01)90223-8"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761988000300019"},{"key":"e_1_3_3_9_1","first-page":"23","volume-title":"Flebotom\u00edneos do Brasil","author":"Galati EAB","year":"2003","unstructured":"Galati EAB. Classifica\u00e7\u00e3o de Phlebotominae. Rangel EF, , Lainson R. 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