{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:41:48Z","timestamp":1789537308127,"version":"build-2803163510"},"reference-count":19,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2012,10]]},"abstract":"\n Disease outbreaks caused by eastern equine encephalitis virus (EEEV; Togaviridae,\n Alphavirus<\/jats:italic>\n ) may be prevented by implementing effective surveillance and intervention strategies directed against the mosquito vector. Methods for EEEV detection in mosquitoes include a real-time reverse transcriptase PCR technique (TaqMan assay), but we report its failure to detect variants isolated in Connecticut in 2011, due to a single base-pair mismatch in the probe-binding site. To improve the molecular detection of EEEV, we developed a multi-target TaqMan assay by adding a second primer\/probe set to provide redundant targets for EEEV detection. The multi-target TaqMan assay had similar performance characteristics to the conventional assay, but also detected newly-evolving strains of EEEV. The approach described here increases the reliability of the TaqMan assay by creating back-up targets for virus detection without sacrificing sensitivity or specificity.\n <\/jats:p>","DOI":"10.1089\/vbz.2012.1008","type":"journal-article","created":{"date-parts":[[2012,7,26]],"date-time":"2012-07-26T12:34:41Z","timestamp":1343306081000},"page":"872-876","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["Development of a Multi-Target TaqMan Assay to Detect Eastern Equine Encephalitis Virus Variants in Mosquitoes"],"prefix":"10.1177","volume":"12","author":[{"given":"Philip M.","family":"Armstrong","sequence":"first","affiliation":[{"name":"Center for Vector Biology and Zoonotic Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholanna","family":"Prince","sequence":"additional","affiliation":[{"name":"Connecticut Department of Public Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore G.","family":"Andreadis","sequence":"additional","affiliation":[{"name":"Center for Vector Biology and Zoonotic Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,10,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.2135-2137.2003"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/35.3.296"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1612.100640"},{"key":"e_1_3_3_5_1","first-page":"2889","article-title":"Detection of infectious virus from field-collected mosquitoes by Vero cell culture assay","author":"Armstrong PM","year":"2011","unstructured":"Armstrong PM, , Andreadis TG, , Finan SL et al. 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