{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:34:36Z","timestamp":1791120876347,"version":"4.1.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Orientia tsutsugamushi<\/jats:italic>\n                    , the etiologic agent of potentially fatal scrub typhus, is characterized by a high antigenic diversity, which complicates the development of a broadly protective vaccine. Efficacy studies in murine and nonhuman primate models demonstrated the DNA vaccine candidate pKarp47, based upon the\n                    <jats:italic toggle=\"yes\">O. tsutsugamushi<\/jats:italic>\n                    Karp 47-kD HtrA protein gene, to be a successful immunoprophylactic against scrub typhus. To characterize 47-kD HtrA protein diversity among human isolates of\n                    <jats:italic toggle=\"yes\">Orientia<\/jats:italic>\n                    , we sequenced the full open reading frame (ORF) of the 47-kD HtrA gene and analyzed the translated amino acid sequences of 17 patient isolates from Thailand (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =13), Laos (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =2), Australia (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =1), and the United Arab Emirates (UAE) (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =1) and 9 reference strains: Karp (New Guinea), Kato (Japan), Ikeda (Japan), Gilliam (Burma), Boryong (Korea), TA763, TH1811 and TH1817 (Thailand), and MAK243 (China). The percentage identity (similarity) of translated amino acid sequences between 16 new isolates and 9 reference strains of\n                    <jats:italic toggle=\"yes\">O. tsutsugamushi<\/jats:italic>\n                    ranged from 96.4% to 100% (97.4% to 100%). However, inclusion of the recently identified\n                    <jats:italic toggle=\"yes\">Orientia chuto<\/jats:italic>\n                    sp. nov. reduced identity (similarity) values to 82.2% to 83.3% (90.4% to 91.4%). These results demonstrate the diversity of\n                    <jats:italic toggle=\"yes\">Orientia<\/jats:italic>\n                    47-kD HtrA among isolates encountered by humans and therefore provide support for the necessity of developing a broadly protective scrub typhus vaccine that takes this diversity into account.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2012.1112","type":"journal-article","created":{"date-parts":[[2013,4,16]],"date-time":"2013-04-16T09:57:07Z","timestamp":1366106227000},"page":"367-375","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":52,"title":["Diversity of the 47-kD HtrA Nucleic Acid and Translated Amino Acid Sequences from 17 Recent Human Isolates of\n                    <i>Orientia<\/i>"],"prefix":"10.1177","volume":"13","author":[{"given":"Ju","family":"Jiang","sequence":"first","affiliation":[{"name":"Naval Medical Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel H.","family":"Paris","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Blacksell","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuntipa","family":"Aukkanit","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul N.","family":"Newton","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]},{"name":"Mahosot Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rattanaphone","family":"Phetsouvanh","sequence":"additional","affiliation":[{"name":"Mahosot Hospital"},{"name":"University of Oxford","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard","family":"Izzard","sequence":"additional","affiliation":[{"name":"Geelong Hospital","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Stenos","sequence":"additional","affiliation":[{"name":"Geelong Hospital","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R.","family":"Graves","sequence":"additional","affiliation":[{"name":"Geelong Hospital","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P.J.","family":"Day","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen L.","family":"Richards","sequence":"additional","affiliation":[{"name":"Naval Medical Research Center"},{"name":"Uniformed Services University of Health Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,6,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1709.100960"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2007.00375.x"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(76)90127-9"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.3.3.4009"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1355.036"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01889.x"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01526-10"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1086\/425365"},{"key":"e_1_3_3_10_1","first-page":"1791","article-title":"Multicenter GeoSentinel analysis of rickettsial diseases in international travelers, 1996\u20132008","volume":"15","author":"Jensenius M","year":"2009","unstructured":"Jensenius M, , Davis X, , von Sonnenburg F, , Schwartz E et al. 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