{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:50:54Z","timestamp":1777877454142,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>\n                    Real-time fluorescence resonance energy transfer (FRET) PCR and melting curve analysis using newly developed fluorophore-labeled hybridization probes were applied for the detection of\n                    <jats:italic toggle=\"yes\">Trichinella spiralis<\/jats:italic>\n                    DNA in muscle of mice following oral inoculation with 300\n                    <jats:italic toggle=\"yes\">T. spiralis<\/jats:italic>\n                    larvae. The developed assay could detect and differentiate\n                    <jats:italic toggle=\"yes\">T. spiralis<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Trichinella papuae<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Trichinella pseudospiralis<\/jats:italic>\n                    DNAs by the different melting temperatures (\n                    <jats:italic toggle=\"yes\">Tm<\/jats:italic>\n                    ). The assay had a detection limit of 5\u00d710\n                    <jats:sup>2<\/jats:sup>\n                    positive control plasmid copies, which was equivalent to 1\u2009ng of\n                    <jats:italic toggle=\"yes\">T. spiralis<\/jats:italic>\n                    DNA spiked into 250\u2009mg of muscle sample. No fluorescence signal was detected when the technique was applied to the DNA of 27 parasites other than\n                    <jats:italic toggle=\"yes\">Trichinella<\/jats:italic>\n                    spp. The assay could detect\n                    <jats:italic toggle=\"yes\">T. spiralis<\/jats:italic>\n                    DNA in muscle at 7, 14, and 21 days postinoculation. The range, mean\u00b1standard deviation, and median of the\n                    <jats:italic toggle=\"yes\">Tm<\/jats:italic>\n                    values of all positive muscle tissue samples were 60.4\u201360.8, 60.6\u00b10.2, and 60.5, respectively. This assay provides an effective tool for the specific, sensitive, and high-throughput detection of\n                    <jats:italic toggle=\"yes\">T. spiralis<\/jats:italic>\n                    DNA in muscle during the early stage of infection. In addition, the technique can be useful for epidemiologic surveillance in naturally infected wildlife.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2012.1221","type":"journal-article","created":{"date-parts":[[2013,6,29]],"date-time":"2013-06-29T09:51:26Z","timestamp":1372499486000},"page":"674-681","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Early Detection of\n                    <i>Trichinella spiralis<\/i>\n                    in Muscle of Infected Mice by Real-Time Fluorescence Resonance Energy Transfer PCR"],"prefix":"10.1177","volume":"13","author":[{"given":"Chairat","family":"Tantrawatpan","sequence":"first","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Thammasat University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Mahasarakham University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penchom","family":"Janwan","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thidarut","family":"Boonmars","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nimit","family":"Morakote","sequence":"additional","affiliation":[{"name":"Chiang Mai University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,9,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.12.009"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200006474X"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-0971-x"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.72.649"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.03.054"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/3283703"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.7.1871-1876.1998"},{"key":"e_1_3_3_9_1","unstructured":"Dupouy-Camet J Murell KD. 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