{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:51:43Z","timestamp":1777877503105,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>\n                    The recent characterization of the 18S ribosomal RNA (rRNA) of a pathogenic\n                    <jats:italic toggle=\"yes\">Babesia<\/jats:italic>\n                    species in a domestic sow paved the way for establishing diagnostic and epidemiological tools for porcine babesiosis. Here, we developed the first specific\n                    <jats:italic toggle=\"yes\">Babesia<\/jats:italic>\n                    sp. Suis PCR, and we applied this test to a panel of samples collected from animals living in a typical Mediterranean environment (Sardinia, Italy), including domestic pigs, wild boars, and ticks. In domestic pigs, PCR coupled with sequencing revealed an estimated\n                    <jats:italic toggle=\"yes\">Babesia<\/jats:italic>\n                    infection frequency of 26.2% and the presence of distinct 18S sequence types. The different distribution of sequence types in symptomatic and asymptomatic subjects might suggest the existence of phylogenetically closely related strains with variable pathogenicity in pigs. Moreover, molecular identification of tick species indicated\n                    <jats:italic toggle=\"yes\">Rhipicephalus sanguineus<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Rhipicephalus bursa<\/jats:italic>\n                    as candidate vectors potentially involved in the transmission of this pathogen. Collectively, the data reveal the suitability of 18S rRNA PCR\/sequencing for molecular diagnosis of porcine babesiosis and for large-scale investigations on the presence and geographical distribution of\n                    <jats:italic toggle=\"yes\">Babesia sp<\/jats:italic>\n                    . Suis genetic variants.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2014.1648","type":"journal-article","created":{"date-parts":[[2014,10,17]],"date-time":"2014-10-17T15:02:34Z","timestamp":1413558154000},"page":"716-723","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Epizootiology and Diagnosis of Porcine Babesiosis in Sardinia, Italy"],"prefix":"10.1177","volume":"14","author":[{"given":"Rosanna","family":"Zobba","sequence":"first","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Maria","family":"Nuvoli","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Sotgiu","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Lecis","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Spezzigu","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Mario","family":"Dore","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco Antonio","family":"Masia","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Cacciotto","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Luisa Pinna","family":"Parpaglia","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Dess\u00ec","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Pittau","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Alberti","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Sassari","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,10,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1108.050085"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1373.055"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.21.10034"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.07.012"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(03)00141-9"},{"key":"e_1_3_3_8_1","first-page":"61","volume-title":"Advances in Zoology Research","author":"Criado-Fornelio A","year":"2012","unstructured":"Criado-Fornelio A. The parasites of wild boar and their impact on conservation: A review. In: Jenkins OP, ed. Advances in Zoology Research, vol. 3. Hauppage, NY: Nova Science Publishers, 2012:61\u2013100."},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2012.12.001"},{"key":"e_1_3_3_10_1","first-page":"39","article-title":"Ticks infesting domestic animals in Italy: Current acarological studies carried out in Sardinia and Basilicata regions","volume":"41","author":"Di Todaro N","year":"1999","unstructured":"Di Todaro N, , Piazza C, , Otranto D, , Giangaspero A. Ticks infesting domestic animals in Italy: Current acarological studies carried out in Sardinia and Basilicata regions. 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