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Target antigens were assembled into a multiplex and tested in serum samples from infected wild-type RVFV or MP12, a modified live virus vaccine. As expected, the N protein was immunodominant and the best target for early detection of infection. Antibody activity against the other targets was also detected. The experimental results demonstrate the capabilities of FMIA for the detection of antibodies to RVFV structural and nonstructural proteins, which can be applied to future development and validation of diagnostic tests that can be used to differentiate vaccinated from infected animals.<\/jats:p>","DOI":"10.1089\/vbz.2014.1721","type":"journal-article","created":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T16:35:28Z","timestamp":1467736528000},"page":"550-557","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine"],"prefix":"10.1177","volume":"16","author":[{"given":"Mohammad M.","family":"Hossain","sequence":"first","affiliation":[{"name":"Kansas State University"},{"name":"Center for Grain and Animal Health Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Center for Grain and Animal Health Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonto","family":"Faburay","sequence":"additional","affiliation":[{"name":"Kansas State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Richt","sequence":"additional","affiliation":[{"name":"Kansas State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"McVey","sequence":"additional","affiliation":[{"name":"Center for Grain and Animal Health Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond R.","family":"Rowland","sequence":"additional","affiliation":[{"name":"Kansas State University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,8,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)74068-X"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(02)00295-9"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.9798-9806.2004"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02501-07"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06046-11"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.02.017"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149174"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01933-06"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.04.043"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-66-10-2271"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00335-11"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.04618904"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001423"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2007.05.006"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700340418"},{"key":"e_1_3_3_17_1","first-page":"941","article-title":"Rainfall and epizootic Rift Valley fever","volume":"63","author":"Davies FG","year":"1985","unstructured":"Davies FG, , Linthicum KJ, , James AD. 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