{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:16:13Z","timestamp":1779912973042,"version":"3.53.1"},"reference-count":26,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Apart from infection with human filariae, zoonotic filariasis also occurs worldwide, and the numbers of cases have been increasing steadily. Diagnosis of intact filariae in tissues or organs depends on histological identification. The morphology of parasites in tissue-embedded sections is poor and shows high levels of homoplasy. Thus, the use of morphological characteristics in taxonomic studies is difficult and may not allow a specific diagnosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Here we report the use of real-time PCR with high-resolution melting analysis (HRM) to detect and identify\n                      <jats:italic toggle=\"yes\">Brugia malayi, Brugia pahangi, Wuchereria bancrofti<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Dirofilaria immitis<\/jats:italic>\n                      in paraffin-embedded sections. Assay specificity was determined using other tissue-dwelling parasites,\n                      <jats:italic toggle=\"yes\">Angiostrongylus cantonensis<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Gnathostoma spinigerum<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Cysticercus cellulosae<\/jats:italic>\n                      . We also developed a quick paraffin removal protocol.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Both human and animal filariae in formalin-fixed paraffin-embedded sections (FFPES) were diagnosed and identified rapidly, whereas other parasites were negative. There was no difference in the melting temperature of products amplified from filarial DNA obtained from unstained FFPES and Hematoxylin &amp; Eosin\u2013stained sections. Therefore, the DNA extraction protocols developed in this study could be used for real-time PCR with HRM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>We report the successful application of a HRM-PCR assay to differentiate four filarial parasites in FFPES, thus providing the pathologist with an effective alternative diagnostic procedure. Furthermore, the quick paraffin removal protocol developed could shorten the duration and number of steps required for paraffin removal using a standard protocol.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2014.1762","type":"journal-article","created":{"date-parts":[[2015,8,14]],"date-time":"2015-08-14T14:39:44Z","timestamp":1439563184000},"page":"473-480","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Rapid Differentiation of Filariae in Unstained and Stained Paraffin-Embedded Sections by a High-Resolution Melting Analysis PCR Assay"],"prefix":"10.1177","volume":"15","author":[{"given":"Sirichit","family":"Wongkamchai","sequence":"first","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjaporn","family":"Mayoon","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namthip","family":"Kanakul","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suporn","family":"Foongladda","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darawan","family":"Wanachiwanawin","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hathai","family":"Nochote","sequence":"additional","affiliation":[{"name":"Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumart","family":"Loymek","sequence":"additional","affiliation":[{"name":"Phikulthong Royal Development Study Center","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,8,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-1784-3"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.44.11.924"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088845"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4741.2011.01188.x"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.07.018"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1301.060920"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.01.006"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.06.007"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP28LAOOKSZSVW"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2009.12.010"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041996"},{"key":"e_1_3_3_13_1","first-page":"183","article-title":"A case of subcutaneous dirofilaria (Nochtiella) repens with microfilaremia originating in Corsica","volume":"87","author":"Nozais JP","year":"1994","unstructured":"Nozais JP, , Bain O, , Gentilini M. A case of subcutaneous dirofilaria (Nochtiella) repens with microfilaremia originating in Corsica. Bull Soc Pathol Exot, 1994; 87:183\u2013185.","journal-title":"Bull Soc Pathol Exot"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.73.895"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.04.003"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.366"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.56.452"},{"key":"e_1_3_3_18_1","first-page":"149","article-title":"Human dirofilariasis due to Dirofilaria (Nochtiella) repens: A review of world literature","volume":"37","author":"Pampiglione S","year":"1995","unstructured":"Pampiglione S, , Canestri Trotti G, , Rivasi F. Human dirofilariasis due to Dirofilaria (Nochtiella) repens: A review of world literature. 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