{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:23:46Z","timestamp":1791285826530,"version":"4.1.0"},"reference-count":46,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec>\n                    <jats:title>Backgrounds:<\/jats:title>\n                    <jats:p>\n                      Lymphatic filariasis is principally caused by\n                      <jats:italic toggle=\"yes\">Wuchereria bancrofti<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Brugia malayi<\/jats:italic>\n                      . The other two filarial nematode species,\n                      <jats:italic toggle=\"yes\">Brugia pahangi<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Dirofilaria immitis<\/jats:italic>\n                      , possibly cause human zoonotic diseases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      We propose the development of a PCR assay linked with DNA pyrosequencing as a rapid tool to identify\n                      <jats:italic toggle=\"yes\">W. bancrofti<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">B. malayi<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">B. pahangi<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">D. immitis<\/jats:italic>\n                      in blood samples and mosquitoes. Primers targeting the fragment of the 5S ribosomal RNA and spliced leader sequences were newly designed and developed to identify these four filarial nematodes. Analytical sensitivity and specificity were evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Pyrosequencing determination of nucleotide variations within 36 nucleotides for\n                      <jats:italic toggle=\"yes\">B. malayi<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">B. pahangi<\/jats:italic>\n                      , and 32 nucleotides for\n                      <jats:italic toggle=\"yes\">W. bancrofti<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">D. immitis<\/jats:italic>\n                      is sufficient for differentiation of those filarial nematodes, and for detection of intraspecies genetic variation of\n                      <jats:italic toggle=\"yes\">B. malayi<\/jats:italic>\n                      . This analysis could detect a single\n                      <jats:italic toggle=\"yes\">B. malayi<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">B. pahangi<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">W. bancrofti<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">D. immitis<\/jats:italic>\n                      microfilaria in blood samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Overall, the PCR-linked pyrosequencing-based method was faster than direct sequencing and less expensive than real-time PCR or direct sequencing. This is the possibility of choice that can be applied in a high-throughput platform for identification and surveillance of reservoirs and vectors infected with lymphatic filaria in endemic areas.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2015.1914","type":"journal-article","created":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T16:51:34Z","timestamp":1458751894000},"page":"326-333","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Pyrosequencing Using SL and 5S rRNA as Molecular Markers for Identifying Zoonotic Filarial Nematodes in Blood Samples and Mosquitoes"],"prefix":"10.1177","volume":"16","author":[{"given":"Oranuch","family":"Sanpool","sequence":"first","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Mahasarakham University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chairat","family":"Tantrawatpan","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Thammasat University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Mahasarakham University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penchom","family":"Janwan","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Walailak University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutchanee","family":"Rodpai","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viraphong","family":"Lulitanond","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piyanan","family":"Taweethavonsawat","sequence":"additional","affiliation":[{"name":"Chulalongkorn University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Khon Kaen University","place":["Thailand"]},{"name":"Khon Kaen University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,5,1]]},"reference":[{"key":"e_1_3_3_2_1","first-page":"504","article-title":"A polymerase chain reaction assay for the survey of bancroftian filariasis","volume":"33","author":"Chansiri K","year":"2002","unstructured":"Chansiri K, , Phantana S. 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