{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T08:05:32Z","timestamp":1779437132022,"version":"3.53.1"},"reference-count":50,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p>\n                    The objective of the present study was to estimate the seroprevalence of\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    spp. in humans and cattle at Sharkia Governorate, Egypt. In addition, identification of\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    spp. in milk samples by PCR and culture with the evaluation of the risk factors associated with\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    spp. seroprevalence in humans were carried out. Overall, the seroprevalence of\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    antibodies in the examined cattle was 23.8%, while in human participants it was 21%. The examination of 205 milk samples using PCR revealed that 6.3% were positive for\n                    <jats:italic toggle=\"yes\">B. abortus<\/jats:italic>\n                    biovar 1 and the results were confirmed by culture methods. Multivariate logistic regression revealed that consumption of unpasteurized dairy products, occupational contact with animals, and knowledge about the disease are risk factors associated with infection in humans. This study documented the endemic status of brucellosis in Egypt. Hygienic measures and increased awareness about the disease are recommended to minimize the spread of infection from animals to humans.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2016.1985","type":"journal-article","created":{"date-parts":[[2016,10,18]],"date-time":"2016-10-18T16:54:00Z","timestamp":1476809640000},"page":"758-764","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Seroprevalence of\n                    <i>Brucella<\/i>\n                    spp. in Cattle, Molecular Characterization in Milk, and the Analysis of Associated Risk Factors with Seroprevalence in Humans, Egypt"],"prefix":"10.1177","volume":"16","author":[{"given":"Mohamed M.","family":"El-Diasty","sequence":"first","affiliation":[{"name":"Animal Health Research Institute","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba A.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Zagazig University","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf E.","family":"Sayour","sequence":"additional","affiliation":[{"name":"Animal Health Research Institute","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma I.","family":"El Hofy","sequence":"additional","affiliation":[{"name":"Benha University","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmaa B.M.B.","family":"Tahoun","sequence":"additional","affiliation":[{"name":"Zagazig University","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleh M.","family":"Shafik","sequence":"additional","affiliation":[{"name":"Animal Health Research Institute","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,12,1]]},"reference":[{"key":"e_1_3_2_2_1","first-page":"16","article-title":"Molecular and serological studies on detection of Brucella species in cattle and buffaloes","volume":"2","author":"Abd Al-Azeem MW","year":"2012","unstructured":"Abd Al-Azeem MW, , Elmalt LM, , El Abdein AEZ, , Sayed HH. 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