{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:36:02Z","timestamp":1791344162269,"version":"4.3.1"},"reference-count":14,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To organize entomological campaigns to trap sand flies in selected regions of Albania and to test them for the presence of existing or new phleboviruses and for leishmania DNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Sand flies were collected in 14 locations from May to October 2014 using three different types of traps. Pools with a maximum of 30 individuals were prepared according to gender, trapping site, and trapping date; they were tested for the presence of (1) phlebovirus RNA with three different PCR systems (2) and\n                      <jats:italic toggle=\"yes\">Leishmania<\/jats:italic>\n                      DNA using two different real-time PCR assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A total of 972 sand flies (568 females, 404 males) were aliquoted to 55 pools. Three pools (in two different regions) were positive for\n                      <jats:italic toggle=\"yes\">Leishmania infantum<\/jats:italic>\n                      . Two pools (Kruje region) were positive for phlebovirus RNA and a 575-nucleotide (nt) colinearized sequence of a novel virus most closely related to but clearly distinct from Tehran virus (16% and 3% divergence at nt and amino acid levels). Next generation sequencing analysis indicated that this virus might be transmitted by either\n                      <jats:italic toggle=\"yes\">Phlebotomus neglectus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Phlebotomus tobbi<\/jats:italic>\n                      , or both vectors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      Visceral leishmaniasis has been clinically recognized in Albania for at least 80 years; however, this is the first time that\n                      <jats:italic toggle=\"yes\">L. infantum<\/jats:italic>\n                      , detected by molecular means, has been reported in sand flies in Albania. At the outset of this study, only Adria virus (\n                      <jats:italic toggle=\"yes\">Salehabad species<\/jats:italic>\n                      ) was recognized in Albania. A novel virus, Balkan virus, was identified and genetic analysis revealed that it belongs to the\n                      <jats:italic toggle=\"yes\">Sandfly fever Naples virus<\/jats:italic>\n                      group containing human pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2016.2002","type":"journal-article","created":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T15:06:05Z","timestamp":1477580765000},"page":"802-806","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":23,"title":["Detection of\n                    <i>Leishmania infantum<\/i>\n                    and a Novel Phlebovirus (Balkan Virus) from Sand Flies in Albania"],"prefix":"10.1177","volume":"16","author":[{"given":"Nazli","family":"Ayhan","sequence":"first","affiliation":[{"name":"UMR \u201cEmergence des Pathologies Virales\u201d (EPV: Aix-Marseille Univ\u2014IRD 190\u2014Inserm 1207\u2014EHESP)","place":["France"]},{"name":"APHM Public Hospitals of Marseille","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enkelejda","family":"Velo","sequence":"additional","affiliation":[{"name":"Institute of Public Health","place":["Albania"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"de Lamballerie","sequence":"additional","affiliation":[{"name":"UMR \u201cEmergence des Pathologies Virales\u201d (EPV: Aix-Marseille Univ\u2014IRD 190\u2014Inserm 1207\u2014EHESP)","place":["France"]},{"name":"APHM Public Hospitals of Marseille","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majlinda","family":"Kota","sequence":"additional","affiliation":[{"name":"Institute of Public Health","place":["Albania"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perparim","family":"Kadriaj","sequence":"additional","affiliation":[{"name":"Institute of Public Health","place":["Albania"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Ozbel","sequence":"additional","affiliation":[{"name":"Ege University","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remi N.","family":"Charrel","sequence":"additional","affiliation":[{"name":"UMR \u201cEmergence des Pathologies Virales\u201d (EPV: Aix-Marseille Univ\u2014IRD 190\u2014Inserm 1207\u2014EHESP)","place":["France"]},{"name":"APHM Public Hospitals of Marseille","place":["France"]},{"name":"King Abdulaziz University","place":["Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Bino","sequence":"additional","affiliation":[{"name":"Institute of Public Health","place":["Albania"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,12,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03027-14"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.07.005"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004519"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8312.2000.tb00207.x"},{"key":"e_1_3_3_6_1","first-page":"294","article-title":"DNA primers for amplification of mitochondrial cytochrome oxidase subunit I from diverse metazoan invertebrates","volume":"3","author":"Folmer O","year":"1994","unstructured":"Folmer O, , Black M, , Hoeh W, , Lutz R, et al. DNA primers for amplification of mitochondrial cytochrome oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol, 1994; 3:294\u2013299.","journal-title":"Mol Mar Biol Biotechnol"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00182-09"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1201-9712(02)90139-6"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5249-5255.2004"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.056614-0"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03371.x"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000814"},{"key":"e_1_3_3_13_1","unstructured":"Velo E Bongiorno G Kadriaj P Myrseli TJ et al. The current status of phlebotomine sand flies in Albania and incrimination of Phlebotomus neglectus (Diptera Psychodidae) as vector of Leishmania infantum. PLoS One In press."},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2005121045"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.583"}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/vbz.2016.2002","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2016.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2016.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T07:15:24Z","timestamp":1779434124000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/vbz.2016.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12]]},"references-count":14,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1089\/vbz.2016.2002"],"URL":"https:\/\/doi.org\/10.1089\/vbz.2016.2002","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12]]}}}