{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T20:59:46Z","timestamp":1778705986777,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p>\n                    Information regarding the infection rate and genotype shifts for Japanese encephalitis virus (JEV) are important for JE vaccine application. In Jiangsu province, China, which is one of the provinces with a high prevalence of JE, JEV infection in swine and mosquitoes in certain cities has only been investigated in 2008\u20132009. Lianyungang City has one of the highest numbers of JE cases in Jiangsu province, and it has a high risk of JEV invasion via migrant birds. JEV infection in vectors in Lianyungang City, which has urban and rural parts, has not been investigated. In 2015\u20132016, we collected mosquitoes in cowsheds with ultraviolet light traps and detected JEV by reverse transcription-polymerase chain reaction (RT-PCR) method in\n                    <jats:italic toggle=\"yes\">Culex tritaeniorhynchus<\/jats:italic>\n                    in Xintan village, Xuzhuang village, and Xiaogaozhuang village in Lianyungang City, China. The proportion of positive pools, which is calculated by the number of infected pools to the total number of pools tested in these villages, were 16.67%, 20.00%, and 4.17%, respectively, and the minimum infection rates, which is calculated as the ratio of the number of positive pools to the total number of mosquitoes tested, were 3.33\u2030, 4.00\u2030, and 0.83\u2030, respectively. Four JEV strains from positive samples were coded as LYG-1, LYG-2, LYG-3, and LYG-4, and the complete E genes were sequenced. Furthermore, the complete genome of LYG-3 was sequenced. The phylogenetic analysis indicated that all the four JEV strains belonged to genotype I-b. This is the first report of genotype I JEV strain in Jiangsu province. The high JEV infection rate in\n                    <jats:italic toggle=\"yes\">Culex tritaeniorhynchus<\/jats:italic>\n                    indicated a high risk of JE reemergence in Lianyungang. The detected JEV strains may have similar antigenicity to that of SA14-14-2 according to molecular characters. These findings suggest that the vaccine can still be effective in Lianyungang.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2016.2086","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T11:50:06Z","timestamp":1494935406000},"page":"503-509","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Japanese Encephalitis Virus Infection Rate and Detection of Genotype I From\n                    <i>Culex tritaeniorhynchus<\/i>\n                    Collected From Jiangsu, China"],"prefix":"10.1177","volume":"17","author":[{"given":"Hongliang","family":"Chu","sequence":"first","affiliation":[{"name":"Beijing Institute of Microbiology and Epidemiology","place":["China"]},{"name":"Jiangsu Provincial Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiming","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongna","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxiao","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Institute of Microbiology and Epidemiology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxia","family":"Guo","sequence":"additional","affiliation":[{"name":"Beijing Institute of Microbiology and Epidemiology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Microbiology and Epidemiology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Hangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minghao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Microbiology and Epidemiology","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,7,1]]},"reference":[{"key":"e_1_3_2_2_1","first-page":"1043","volume-title":"Fields Virology, 4th Ed","author":"Burke DS","year":"2001","unstructured":"Burke DS, , Monath TP. Fields Virology, 4th Ed. Philadelphia: Lippincott Willians & Wilkins, 2001:1043\u20131125."},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004686"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtm.2016.03.007"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1501.080311"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003015"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000624"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1586\/erv.11.7"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-015-3621-4"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-4-8"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001231"},{"key":"e_1_3_2_12_1","article-title":"A spatial and temporal analysis of Japanese encephalitis in mainland China, 1963\u20131975: A period without Japanese encephalitis vaccination","volume":"9","author":"Li XL","year":"2014","unstructured":"Li XL, , Gao XY, , Ren ZP, , Cao YX, et al. A spatial and temporal analysis of Japanese encephalitis in mainland China, 1963\u20131975: A period without Japanese encephalitis vaccination. PLoS One, 2014; 9:e99183.","journal-title":"PLoS One"},{"key":"e_1_3_2_13_1","first-page":"5","article-title":"Pathogenicity-related amino acid on protein E of Japanese encephalitis virus","volume":"15","author":"Li Y","year":"2002","unstructured":"Li Y, , Wakita Z, , Yasui K. Pathogenicity-related amino acid on protein E of Japanese encephalitis virus. Chin J Biol, 2002; 15:5\u20138.","journal-title":"Chin J Biol"},{"key":"e_1_3_2_14_1","first-page":"507","article-title":"Epidemiological characteristics of Japanese encephalitis in Shanghai","volume":"15","author":"Li YT","year":"2009","unstructured":"Li YT, , Zhu YY, , Jin BH. Epidemiological characteristics of Japanese encephalitis in Shanghai. Zhongguo Yi Miao He Mian Yi, 2009; 15:507\u2013510, 561.","journal-title":"Zhongguo Yi Miao He Mian Yi"},{"key":"e_1_3_2_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.4.2600-2606.2003"},{"key":"e_1_3_2_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79797-0"},{"key":"e_1_3_2_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004936"},{"key":"e_1_3_2_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/375291a0"},{"key":"e_1_3_2_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055165"},{"key":"e_1_3_2_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra030476"},{"key":"e_1_3_2_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-449"},{"key":"e_1_3_2_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.04.052"},{"key":"e_1_3_2_23_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.242"},{"key":"e_1_3_2_24_1","first-page":"435","article-title":"Epidemiology of Japanese encephalitis: Past, present, and future prospects","volume":"11","author":"Wang H","year":"2015","unstructured":"Wang H, , Liang G. Epidemiology of Japanese encephalitis: Past, present, and future prospects. Ther Clin Risk Manag, 2015; 11:435\u2013448.","journal-title":"Ther Clin Risk Manag"},{"key":"e_1_3_2_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82185-0"},{"key":"e_1_3_2_26_1","first-page":"302","article-title":"Dynamic tendency of Japanese B encephalitis in China","volume":"10","author":"Wang XJ","year":"2004","unstructured":"Wang XJ, , Lu Y, , Zhang YP, , Chen YS, et al. Dynamic tendency of Japanese B encephalitis in China. Chin J Vaccines Immunization, 2004; 10:302\u2013304.","journal-title":"Chin J Vaccines Immunization"},{"key":"e_1_3_2_27_1","unstructured":"WHO.. 2015 www.who.int\/mediacentre\/factsheets\/fs386\/en\/"},{"key":"e_1_3_2_28_1","first-page":"5126","article-title":"The community composition of Avian Fauna at the Coastal Areas in Nantong and Lianyungang","volume":"51","author":"Wu JF","year":"2012","unstructured":"Wu JF, , Qiao JS, , Tian M, , Feng ZJ. The community composition of Avian Fauna at the Coastal Areas in Nantong and Lianyungang. Hubei Agric Sci, 2012; 51:5126\u20135130.","journal-title":"Hubei Agric Sci"},{"key":"e_1_3_2_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307776200"},{"key":"e_1_3_2_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-016-2814-y"},{"key":"e_1_3_2_31_1","first-page":"1146","article-title":"Study on the genotypic characteristics of Japanese encephalitis virus and serology in the healthy population of Zhejiang province, 2012\u20132013","volume":"35","author":"Yan J","year":"2014","unstructured":"Yan J, , Pan J, , Zhang Y, , Zhou J, et al. Study on the genotypic characteristics of Japanese encephalitis virus and serology in the healthy population of Zhejiang province, 2012\u20132013. Zhonghua Liu Xing Bing Xue Za Zhi, 2014; 35:1146\u20131150.","journal-title":"Zhonghua Liu Xing Bing Xue Za Zhi"},{"key":"e_1_3_2_32_1","article-title":"Isolation and genetic characteristics of human genotype 1 Japanese encephalitis virus, China, 2009","volume":"6","author":"Zhang JS","year":"2011","unstructured":"Zhang JS, , Zhao QM, , Guo XF, , Zuo SQ, et al. Isolation and genetic characteristics of human genotype 1 Japanese encephalitis virus, China, 2009. PLoS One, 2011; 6:e16418.","journal-title":"PLoS One"},{"key":"e_1_3_2_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1710"}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/vbz.2016.2086","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2016.2086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2016.2086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:56:04Z","timestamp":1777589764000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/vbz.2016.2086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1089\/vbz.2016.2086"],"URL":"https:\/\/doi.org\/10.1089\/vbz.2016.2086","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]}}}