{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:10:32Z","timestamp":1790489432440,"version":"4.1.0"},"reference-count":52,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Francisella tularensis<\/jats:italic>\n                    causes a highly infectious zoonotic disease tularemia. Both\n                    <jats:italic toggle=\"yes\">Haemaphysalis longicornis<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Hyalomma asiaticum<\/jats:italic>\n                    are widely distributed in China, but the presence of\n                    <jats:italic toggle=\"yes\">Francisella<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Francisella<\/jats:italic>\n                    -like endosymbionts (FLEs) in the two tick species is poorly understood. Therefore, a total of 627\n                    <jats:italic toggle=\"yes\">H<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">longicornis<\/jats:italic>\n                    (471 adults and 156 nymphs) and 88\n                    <jats:italic toggle=\"yes\">Hy. asiaticum<\/jats:italic>\n                    ticks (adults) were collected, of which 88 were from Bole of Xinjiang, 236 from Liaoyang, and 176 from Shenyang of Liaoning, and 215 from Wuhan of Hubei. Notably, five\n                    <jats:italic toggle=\"yes\">H. longicornis<\/jats:italic>\n                    pools from Liaoyang of Liaoning province might have harbored\n                    <jats:italic toggle=\"yes\">F. tularensis<\/jats:italic>\n                    , showing a minimum prevalence of 2.12% (5\/236). This study should alert the health department and veterinarians working within the region to prevent and control the emergence of tularemia. After the screening of 16S rRNA and\n                    <jats:italic toggle=\"yes\">tul4<\/jats:italic>\n                    genes, the results revealed that FLEs were detected in\n                    <jats:italic toggle=\"yes\">Hy. asiaticum<\/jats:italic>\n                    ticks in Bole and in\n                    <jats:italic toggle=\"yes\">H. longicornis<\/jats:italic>\n                    ticks in Liaoyang and Shenyang. Their infection rate was 100% (88\/88), 3.39% (8\/236 is a minimum), and 8.52% (15\/176), respectively. Phylogenetic analyses indicated that the sequence named bole in\n                    <jats:italic toggle=\"yes\">Hy. Asiaticum<\/jats:italic>\n                    from Bole, the sequence named liaoyang1 in\n                    <jats:italic toggle=\"yes\">H. longicornis<\/jats:italic>\n                    from Liaoyang, and the sequence named shanyang1 in\n                    <jats:italic toggle=\"yes\">H. longicornis<\/jats:italic>\n                    from Shenyang shared consistent 16S rRNA sequence, and the difference between Chinese FLEs and the known FLEs was obvious. These findings suggest that this FLE species might be a potentially novel FLE circulating in\n                    <jats:italic toggle=\"yes\">H. longicornis<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Hy. asiaticum<\/jats:italic>\n                    from China.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2017.2252","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T14:44:24Z","timestamp":1534257864000},"page":"669-676","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["A Novel\n                    <i>Francisella<\/i>\n                    -Like Endosymbiont in\n                    <i>Haemaphysalis longicornis<\/i>\n                    and\n                    <i>Hyalomma asiaticum<\/i>\n                    , China"],"prefix":"10.1177","volume":"18","author":[{"given":"Yanhua","family":"Wang","sequence":"first","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Mao","sequence":"additional","affiliation":[{"name":"Institute for the Prevention and Control of Infections and Infectious Diseases","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingwei","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute for the Prevention and Control of Infections and Infectious Diseases","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute for the Prevention and Control of Infections and Infectious Diseases","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Vector Biological Control Institute","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jibo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Vector Biological Control Institute","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Peng","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianxu","family":"Xia","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,11,28]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-220"},{"key":"e_1_3_4_3_1","first-page":"304","article-title":"A case of ulceroglandular tularemia","volume":"57","author":"Ahn WS","year":"1999","unstructured":"Ahn WS, , Oh MG, , Lee JH. A case of ulceroglandular tularemia. 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