{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:54:12Z","timestamp":1777877652909,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Leishmania mexicana<\/jats:italic>\n                    is an intracellular parasite that causes cutaneous leishmaniasis (CL) in some countries, including Mexico. Recently, we identified the elongation factor-1\u03b1 (EF-1\u03b1) of\n                    <jats:italic toggle=\"yes\">L. mexicana<\/jats:italic>\n                    by immunoproteomic analysis. In\n                    <jats:italic toggle=\"yes\">Leishmania donovani<\/jats:italic>\n                    , this molecule has been reported as a virulence factor involved in downregulation of macrophages by no-canonical function when EF-1\u03b1 interacts with protein tyrosine phosphatase-1 (SHP-1). However, in\n                    <jats:italic toggle=\"yes\">L. mexicana<\/jats:italic>\n                    the key role of EF-1\u03b1 in host\u2013parasite relationship has not been elucidated, by this reason we started with cloning and recombinant expression of this antigen. A sequence of 1350\u2009bp corresponding to\n                    <jats:italic toggle=\"yes\">EF-1\u03b1<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">EF-Lm<\/jats:italic>\n                    ) full-length gene was amplified from genomic DNA of\n                    <jats:italic toggle=\"yes\">L. mexicana<\/jats:italic>\n                    (GenBank: MG256973); this gene contains only one nucleotide change: C464T, compared with\n                    <jats:italic toggle=\"yes\">L. mexicana<\/jats:italic>\n                    reference sequence (GenBank: FR799570.1). The gene cloned (\n                    <jats:italic toggle=\"yes\">EF-Lm<\/jats:italic>\n                    ) codes for a protein of 449 residues. It was expressed in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    and purified as 63\u2009kDa sumo-fusion protein, which was recognized in the sera of patients diagnosed with CL. Our results show that EF-Lm is immunogenic during infection, and the rEF-Lm could be used for further analyses in the host\u2013parasite relationship.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2018.2347","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T16:07:37Z","timestamp":1538064457000},"page":"709-712","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Cloning and Recombinant Expression of Elongation Factor-1\u03b1 of\n                    <i>Leishmania mexicana<\/i>"],"prefix":"10.1177","volume":"18","author":[{"given":"Kenia","family":"L\u00f3pez-L\u00f3pez","sequence":"first","affiliation":[{"name":"Universidad Aut\u00f3noma de Sinaloa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annete I.","family":"Apodaca-Medina","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Sinaloa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia R.","family":"Le\u00f3n-Sicairos","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Sinaloa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina C.","family":"Mur\u00faa-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Sinaloa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vianney F.","family":"Ortiz-Navarrete","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanett","family":"Ch\u00e1vez-Ontiveros","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Sinaloa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelina","family":"Beltr\u00e1n-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Sinaloa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9ctor S.","family":"L\u00f3pez-Moreno","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Sinaloa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,11,28]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002322"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.053"},{"key":"e_1_3_3_4_1","article-title":"Evaluation of Leishmania donovani protein disulfide isomerase as potential immunogenic protein\/vaccine candidate against visceral leishmaniasis","volume":"7","author":"Kushawaha P","year":"2012","unstructured":"Kushawaha P, , Gupta R, , Tripathi C, , Sundar S. Evaluation of Leishmania donovani protein disulfide isomerase as potential immunogenic protein\/vaccine candidate against visceral leishmaniasis. PLoS One, 2012; 7:e35670.","journal-title":"PLoS One"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.03.077"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2003.00658.x"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00216-X"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2011.0688"},{"key":"e_1_3_3_9_1","first-page":"433","article-title":"Cutaneous leishmaniasis caused by Leishmania mexicana in Durango, M\u00e9xico: First clinical case report","volume":"145","author":"P\u00e9rez-Vega J","year":"2009","unstructured":"P\u00e9rez-Vega J, , L\u00f3pez-Moreno C, , L\u00f3pez-Valenzuela J, , Rend\u00f3n-Maldonado J, et al. Cutaneous leishmaniasis caused by Leishmania mexicana in Durango, M\u00e9xico: First clinical case report. Gac Med Mex, 2009; 145:433\u2013435.","journal-title":"Gac Med Mex"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2014.1712"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-36342010000200009"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2011.01.006"}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/vbz.2018.2347","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2018.2347","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2018.2347","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:57:57Z","timestamp":1777589877000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/vbz.2018.2347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,28]]},"references-count":11,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1089\/vbz.2018.2347"],"URL":"https:\/\/doi.org\/10.1089\/vbz.2018.2347","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,28]]}}}