{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:57:25Z","timestamp":1790582245205,"version":"4.1.0"},"reference-count":30,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:p>\n                    Leptospirosis is a worldwide zoonosis caused by spirochetes from the genus\n                    <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                    . In the present study, a visual and rapid method for detecting\n                    <jats:italic toggle=\"yes\">Leptospira interrogans<\/jats:italic>\n                    was developed based on multiple cross-displacement amplification (MCDA) and nanoparticle-based lateral flow biosensor (LFB). A set of 10 primers was specifically designed to recognize 10 regions of the\n                    <jats:italic toggle=\"yes\">lipL 41<\/jats:italic>\n                    gene of\n                    <jats:italic toggle=\"yes\">L. interrogans<\/jats:italic>\n                    . The MCDA reaction was optimized at 64\u00b0C for only 40\u2009min, and the amplification products were directly applied to the biosensor. The entire process, including DNA extraction (25\u2009min), MCDA reaction (40\u2009min), and result interpretation (\u223c2\u2009min), could be completed within 70\u2009min. Amplification products were detectable from as few as 10 genomic equivalents per reaction of pure\n                    <jats:italic toggle=\"yes\">L. interrogans<\/jats:italic>\n                    DNA. No cross-reaction with nonpathogenic\n                    <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                    or other bacteria was observed. The MCDA-LFB method established in the current report is suitable for the rapid screening of\n                    <jats:italic toggle=\"yes\">L. interrogans<\/jats:italic>\n                    in clinical, animal, and environmental samples.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2018.2395","type":"journal-article","created":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T10:48:56Z","timestamp":1548931736000},"page":"604-612","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Visual and Rapid Detection of\n                    <i>Leptospira interrogans<\/i>\n                    Using Multiple Cross-Displacement Amplification Coupled with Nanoparticle-Based Lateral Flow Biosensor"],"prefix":"10.1177","volume":"19","author":[{"given":"Shijun","family":"Li","sequence":"first","affiliation":[{"name":"Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Chen","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Wang","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilin","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, College of Medicine, Zhejiang University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, College of Medicine, Zhejiang University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,8,2]]},"reference":[{"key":"e_1_3_4_2_1","first-page":"1","article-title":"History of leptospirosis and leptospira","volume":"387","author":"Adler B","year":"2015","unstructured":"Adler B. 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