{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:53:12Z","timestamp":1790351592735,"version":"4.1.0"},"reference-count":18,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T00:00:00Z","timestamp":1580428800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:p>\n                    Murine typhus is a tropical disease caused\n                    <jats:italic toggle=\"yes\">by Rickettsia typhi<\/jats:italic>\n                    and is endemic in resource-limited settings such as Southeast Asian countries. Early diagnosis of\n                    <jats:italic toggle=\"yes\">R. typhi<\/jats:italic>\n                    infection facilitates appropriate management and reduces the risk of severe disease. However, molecular detection of\n                    <jats:italic toggle=\"yes\">R. typhi<\/jats:italic>\n                    in blood is insensitive due to low rickettsemia. Furthermore, the gold standard of sero-diagnosis by immunofluorescence assay (IFA) is cumbersome, subjective, impractical, and unavailable in many endemic areas. In an attempt to identify a practical diagnostic approach that can be applied in Indonesia, we evaluated the performance of commercial\n                    <jats:italic toggle=\"yes\">R. typhi<\/jats:italic>\n                    IgM and IgG enzyme-linked immunosorbent assay (ELISA) and IFA using paired plasma from previously studied\n                    <jats:italic toggle=\"yes\">R. typhi<\/jats:italic>\n                    PCR-positive cases and controls with other known infections. Sensitivity and specificity of combined ELISA IgM and IgG anti-\n                    <jats:italic toggle=\"yes\">R. typhi<\/jats:italic>\n                    using paired specimens were excellent (95.0% and 98.3%, respectively), comparable to combined IFA IgM and IgG (97.5% and 100%, respectively); sensitivity of ELISA IgM from acute specimens only was poor (45.0%), but specificity was excellent (98.3%). IFA IgM was more sensitive (77.5%), but less specific (89.7%) for single specimens.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2019.2451","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T10:23:51Z","timestamp":1566815031000},"page":"93-99","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Comparison of Commercial Enzyme-Linked Immunosorbent Assay and Immunofluorescence Assay for Diagnosis of Acute\n                    <i>Rickettsia typhi<\/i>\n                    Infections"],"prefix":"10.1177","volume":"20","author":[{"given":"Dewi","family":"Lokida","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology, Tangerang District Hospital, Tangerang, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratiwi","family":"Sudarmono","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universitas Indonesia, Depok, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman","family":"Kosasih","sequence":"additional","affiliation":[{"name":"Indonesia Research Partnership on Infectious Disease (INA-RESPOND), National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deni Pepy","family":"Butar-butar","sequence":"additional","affiliation":[{"name":"Indonesia Research Partnership on Infectious Disease (INA-RESPOND), National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustiani","family":"Salim","sequence":"additional","affiliation":[{"name":"Indonesia Research Partnership on Infectious Disease (INA-RESPOND), National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ungke","family":"Antonjaya","sequence":"additional","affiliation":[{"name":"Eijkman-Oxford Clinical Research Unit, Jakarta, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rizky Amalia","family":"Sari","sequence":"additional","affiliation":[{"name":"Indonesia Research Partnership on Infectious Disease (INA-RESPOND), National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abu Tholib","family":"Aman","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Parwati","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Universitas Padjadjaran\/Hasan Sadikin Hospital, Bandung, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansyur","family":"Arif","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universitas Hasanudin\/Dr. Wahidin Sudirohusodo Hospital, Makassar, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuen-Yen","family":"Lau","sequence":"additional","affiliation":[{"name":"Collaborative Clinical Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Karyana","sequence":"additional","affiliation":[{"name":"Indonesia Research Partnership on Infectious Disease (INA-RESPOND), National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia."},{"name":"National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,1,31]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0191"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr6502a1"},{"key":"e_1_3_3_4_1","volume-title":"Rickettsiales: Laboratory Diagnosis. S. Thomas","author":"Blanton LS","year":"2016","unstructured":"Blanton LS. Rickettsiales: Laboratory Diagnosis. S. Thomas. 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