{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:54:52Z","timestamp":1777877692664,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>\n                    Dengue virus (DENV), a member of the family\n                    <jats:italic toggle=\"yes\">Flaviviridae<\/jats:italic>\n                    , is the causative agent of dengue fever, the most prevalent mosquito-borne viral illness in humans, representing a major public health concern in the tropical countries. Although humoral immunity to DENV has been extensively studied and widely used, little is known about the potential diagnostic use of T cell response for DENV diagnosis. The aim of our study was to characterize the T cell immunity in subjects with acute or past DENV infection, using an original and easy to perform\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    ELISpot assay, and to evaluate the role of cross-reactivity between the four DENV serotypes and between DENV and Zika virus (ZIKV). As controls, DENV-seronegative healthy subjects were enrolled and a cutoff of positive DENV-specific T cell response was calculated. DENV-specific T cell response for at least one DENV serotype was detected among all DENV-specific neutralization positive subject. Furthermore, our data showed that in acute DENV infection, the DENV-specific effector memory T cell response against the relevant serotype was predominant. However, a high level of cross-reactivity among all DENV serotypes was also documented. DENV-specific T cell response was almost undetectable among DENV-seronegative subjects with ZIKV acute infection, supporting the hypothesis that the assay could be useful in differential diagnosis between ZIKV and DENV infection.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2019.2515","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T16:35:28Z","timestamp":1575995728000},"page":"295-302","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Dengue Virus-Specific Humoral and T Cellular Immune Response in Italian Residents and Travelers Returning from Endemic Areas"],"prefix":"10.1177","volume":"20","author":[{"given":"Irene","family":"Cassaniti","sequence":"first","affiliation":[{"name":"Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Percivalle","sequence":"additional","affiliation":[{"name":"Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kodjo Messan Guy","family":"Adzasehoun","sequence":"additional","affiliation":[{"name":"Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuditta","family":"Comolli","sequence":"additional","affiliation":[{"name":"Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."},{"name":"Experimental Research Laboratories, Biotechnology Area, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fausto","family":"Baldanti","sequence":"additional","affiliation":[{"name":"Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."},{"name":"Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,3,31]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00069-07"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2011.03.005"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010867107"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2009.11.001"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00330-07"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan6836"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1408.080287"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00252-16"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/pgmj.2004.019638"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020581"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm887"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737699"},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit273"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1607931113"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02675-12"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.5.1922-1927.2001"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.09.103"},{"key":"e_1_3_5_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf8505"},{"key":"e_1_3_5_20_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.12-0461"},{"key":"e_1_3_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2013.05.014"},{"key":"e_1_3_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.02.003"},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305227110"},{"key":"e_1_3_5_24_1","first-page":"456","article-title":"Identifying candidate targets of immune responses in Zika virus based on homology to epitopes in other flavivirus species","volume":"23","author":"Xu X","year":"2016","unstructured":"Xu X, , Vaughan K, , Weiskopf D, , Grifoni A, et al. Identifying candidate targets of immune responses in Zika virus based on homology to epitopes in other flavivirus species. Nat Struct Mol Biol, 2016; 23:456\u2013458.","journal-title":"Nat Struct Mol Biol"},{"key":"e_1_3_5_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1306333110"}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/vbz.2019.2515","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2019.2515","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2019.2515","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:59:14Z","timestamp":1777589954000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/vbz.2019.2515"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,31]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1089\/vbz.2019.2515"],"URL":"https:\/\/doi.org\/10.1089\/vbz.2019.2515","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,31]]}}}