{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:54:56Z","timestamp":1777877696801,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T00:00:00Z","timestamp":1580428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T00:00:00Z","timestamp":1580428800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Coxiella burnetii<\/jats:italic>\n                    is an obligate intracellular pathogen and the causative agent of Q fever. In Ukraine, 28 human cases of Q fever were reported between 1997 and 2006; however, there are no state-approved, standardized molecular diagnostic assays that can be used systematically to investigate\n                    <jats:italic toggle=\"yes\">C. burnetii<\/jats:italic>\n                    transmission to humans and its distribution throughout Ukraine. To address this deficiency, we followed the recommendation of the World Organization for Animal Health (OIE) and developed a confirmatory PCR for\n                    <jats:italic toggle=\"yes\">C. burnetii<\/jats:italic>\n                    for veterinary diagnosis in Ukraine. The PCR assay targeted the outer membrane-associated gene com1 in\n                    <jats:italic toggle=\"yes\">C. burnetii<\/jats:italic>\n                    . Oligonucleotide primers were selected that amplify a 689-bp DNA fragment of the\n                    <jats:italic toggle=\"yes\">com1<\/jats:italic>\n                    gene (primers: CoxF2\u2009=\u20095\u2032-ACYGCAGGCGTGGCGATAG-3\u2032 and CoxR4\u2009=\u20095\u2032-TGAAGGTTTTGTTGTGAGGTGGC-3\u2032). The assay proved highly sensitive and specific to\n                    <jats:italic toggle=\"yes\">C. burnetii<\/jats:italic>\n                    DNA detection (LOD\u2009=\u20090.37\u2009pg\/\u03bcL). Reproducibility of the test was verified by comparing the PCR results with those of a different PCR protocol and qPCR. Using the CoxF2\/CoxR4 primer set and reaction conditions described here, the PCR Diagnostic Kit\n                    <jats:italic toggle=\"yes\">C. burnetii-<\/jats:italic>\n                    PCR-TEST was developed and officially registered for use in Ukraine by the State Scientific Control Institute of Biotechnology and Strains (Kyiv, Ukraine) for diagnostic purposes.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2019.2518","type":"journal-article","created":{"date-parts":[[2019,9,19]],"date-time":"2019-09-19T14:06:28Z","timestamp":1568901988000},"page":"100-106","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of a PCR Kit for Detection of\n                    <i>Coxiella burnetii<\/i>\n                    in Ukraine"],"prefix":"10.1177","volume":"20","author":[{"given":"Lyudmyla V.","family":"Marushchak","sequence":"first","affiliation":[{"name":"Research Virology Department, State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oleg N.","family":"Deriabin","sequence":"additional","affiliation":[{"name":"State Scientific Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liudmyla","family":"Dedok","sequence":"additional","affiliation":[{"name":"Research Virology Department, State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Volosyanko","sequence":"additional","affiliation":[{"name":"Ukrainian Laboratory of Quality and Safety of Agriculture Products of the National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana","family":"Garcavenko","sequence":"additional","affiliation":[{"name":"Research Virology Department, State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,1,31]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-38"},{"key":"e_1_3_4_3_1","first-page":"81","article-title":"Q fever: A zoonosis","volume":"5","author":"Babudieri B","year":"1959","unstructured":"Babudieri B. Q fever: A zoonosis. Adv Vet Sci, 1959; 5:81\u2013182.","journal-title":"Adv Vet Sci"},{"key":"e_1_3_4_4_1","first-page":"33","article-title":"Clinical and epidemiologic manifestations of Q-fever in the area between the Danube-Dniester Ukraine. Collection of scientific works of the 12th Annual Scientific Practical Conference with international participation timed to the Science Day the, 75th anniversary of the Institute, 105th anniversary H. Mosing. Modern problems of epidemiology, microbiology, hygiene and of tuberculosis","volume":"2","author":"Beck NG","year":"2015","unstructured":"Beck NG, , Kushnir ZG, , Lutsyk TS, , Goncharov VA, et al. Clinical and epidemiologic manifestations of Q-fever in the area between the Danube-Dniester Ukraine. Collection of scientific works of the 12th Annual Scientific Practical Conference with international participation timed to the Science Day the, 75th anniversary of the Institute, 105th anniversary H. Mosing. Modern problems of epidemiology, microbiology, hygiene and of tuberculosis. Lviv, 2015; 2:33\u201338.","journal-title":"Lviv"},{"key":"e_1_3_4_5_1","first-page":"143","volume-title":"Infectious Animal Diseases","author":"Bessarabov BF","year":"2007","unstructured":"Bessarabov BF, , Vashutin AA, , Voronin ES. [Infectious Animal Diseases] Moscow, Russia: Kolos, 2007:143\u2013150 (Article in Russian)."},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05097-11"},{"key":"e_1_3_4_7_1","unstructured":"Bruin de A Janse I Rotterdam van BJ. RIVM letter report 330291002: Molecular detection and typing of Coxiella burnetii. 2009:1\u201327. Available at www.rivm.nl\/bibliotheek\/rapporten\/330291002.pdf"},{"key":"e_1_3_4_8_1","first-page":"21","article-title":"EFSA panel on animal health and welfare (AHAW); scientific opinion on Q fever","volume":"8","author":"European Food Safety Authority (EFSA)","year":"2010","unstructured":"European Food Safety Authority (EFSA). EFSA panel on animal health and welfare (AHAW); scientific opinion on Q fever. EFSA J, 2010a; 8:21\u201323.","journal-title":"EFSA J"},{"key":"e_1_3_4_9_1","first-page":"241","article-title":"The community summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008","volume":"8","author":"European Food Safety Authority (EFSA)","year":"2010","unstructured":"European Food Safety Authority (EFSA). The community summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008. EFSA J, 2010b; 8:241\u2013246.","journal-title":"EFSA J"},{"key":"e_1_3_4_10_1","first-page":"228","article-title":"Scientific Report of EFSA and ECDC. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012","volume":"12","author":"European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA\/ECDC)","year":"2014","unstructured":"European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA\/ECDC). Scientific Report of EFSA and ECDC. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA J, 2014; 12:228\u2013240.","journal-title":"EFSA J"},{"key":"e_1_3_4_11_1","first-page":"110","article-title":"The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013","volume":"13","author":"EFSA EC","year":"2015","unstructured":"European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA and ECDC). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013. EFSA J, 2015; 13:110\u2013112.","journal-title":"EFSA J"},{"key":"e_1_3_4_12_1","first-page":"164","article-title":"The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015","volume":"14","author":"European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA\/ECDC)","year":"2016","unstructured":"European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA\/ECDC). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015. 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Collection of scientific works of the 12th Annual Scientific Practical Conference with international participation timed to the Science Day the, 75th anniversary of the Institute, 105th anniversary H. Mosing. Modern problems of epidemiology, microbiology, hygiene and of tuberculosis","volume":"12","author":"Kushnir ZG","year":"2015","unstructured":"Kushnir ZG, , Lutsyk TS, , Bek NG, , Bilous OA, et al. Study of Coxiella Burnetii distribution in the absence of Q fever diseases. Collection of scientific works of the 12th Annual Scientific Practical Conference with international participation timed to the Science Day the, 75th anniversary of the Institute, 105th anniversary H. Mosing. Modern problems of epidemiology, microbiology, hygiene and of tuberculosis. Lviv, 2015; 12:41\u201347.","journal-title":"Lviv"},{"key":"e_1_3_4_19_1","first-page":"44","article-title":"Urgency and modern methodological workings out antigenic preparations for diagnostics of tick-borne spotted fever in Ukraine. Collection of scientific works of the 12th Annual Scientific Practical Conference with international participation timed to the Science Day the, 75th anniversary of the Institute, 105th anniversary H. Mosing. Modern problems of epidemiology, microbiology, hygiene and of tuberculosis","volume":"12","author":"Loginov JA","year":"2015","unstructured":"Loginov JA, , Kushnir ZG, , Zarichna OZ, , Vsyaka IV. Urgency and modern methodological workings out antigenic preparations for diagnostics of tick-borne spotted fever in Ukraine. Collection of scientific works of the 12th Annual Scientific Practical Conference with international participation timed to the Science Day the, 75th anniversary of the Institute, 105th anniversary H. Mosing. Modern problems of epidemiology, microbiology, hygiene and of tuberculosis. Lviv, 2015; 12:44\u201347.","journal-title":"Lviv"},{"key":"e_1_3_4_20_1","first-page":"257","article-title":"Method for detection and identification of the Q fever by polymerase chain reaction (PCR)","volume":"23","author":"Marushchak LV","year":"2013","unstructured":"Marushchak LV. Method for detection and identification of the Q fever by polymerase chain reaction (PCR). Bull Vet Biotechnol, 2013; 23:257\u2013263.","journal-title":"Bull Vet Biotechnol"},{"key":"e_1_3_4_21_1","first-page":"1","article-title":"Q-fever: Epizootology and laboratory diagnostics (PhD Thesis)","author":"Marushchak LV","year":"2016","unstructured":"Marushchak LV. Q-fever: Epizootology and laboratory diagnostics (PhD Thesis). 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