{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:55:22Z","timestamp":1777877722458,"version":"3.51.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T00:00:00Z","timestamp":1601337600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>\n                    Japanese encephalitis virus (JEV) is recognized as a public health risk by the World Health Organization. In Asia, each year, \u223c70,000 people become infected with JEV, which results in \u223c10,000 deaths. Chikungunya virus (CHIKV) is an RNA virus, whose infection mainly causes fever, myalgia, and skin rash. Although the mortality rate is low, it seriously affects daily life. JEV and CHIKV infect humans through mosquitoes; therefore, a recombinant vaccinia virus coexpressing JEV E and CHIKV E1 proteins was constructed to prevent their concurrent infection. In this study, after mice first immunization, booster immunization was performed at 21 days postimmunization (dpi). At 35\u2009dpi, mice were challenged with JEV and CHIKV. Specific antibodies significantly increased in the rVTT-CE1-JE-EGFP group, which were significantly (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01) higher than those of the control groups at 35\u2009dpi. The plaque reduction neutralization tests (JEV) of rVTT-CE1-JE-EGFP group was 1:320 at 35\u2009dpi. Furthermore, cytokine levels and the percentage of CD3\n                    <jats:sup>+<\/jats:sup>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD3\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T-lymphocytes in the rVTT-CE1-JE-EGFP group were significantly (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01) higher than those in the control groups at 35\u2009dpi. After challenge, mice body weights in rVTT-CE1-JE-EGFP group were not significantly altered, and the survival rate was 100%. These results showed the rVTT-CE1-JE-EGFP group elicited significant humoral and cellular immune responses, thus indicating that the recombinant vaccine may serve as a candidate for effective prevention of CHIKV and JEV infection.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2020.2613","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T16:45:21Z","timestamp":1593103521000},"page":"788-796","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Construction and Immunogenicity of Recombinant Vaccinia Virus Vaccine Against Japanese Encephalitis and Chikungunya Viruses Infection in Mice"],"prefix":"10.1177","volume":"20","author":[{"given":"Ying","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, College of Animal Science, Jilin University, Changchun, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji-cheng","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Military Veterinary, Academy of Military Sciences, Changchun, People's Republic of China."},{"name":"Changchun University of Chinese Medicine, Changchun, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Jing","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, College of Animal Science, Jilin University, Changchun, People's Republic of China."},{"name":"Institute of Military Veterinary, Academy of Military Sciences, Changchun, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Engineering, Foshan University, Foshan, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Military Veterinary, Academy of Military Sciences, Changchun, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao-hui","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, College of Animal Science, Jilin University, Changchun, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning-yi","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, College of Animal Science, Jilin University, Changchun, People's Republic of China."},{"name":"Institute of Military Veterinary, Academy of Military Sciences, Changchun, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-jun","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Military Veterinary, Academy of Military Sciences, Changchun, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,9,29]]},"reference":[{"key":"e_1_3_4_2_1","volume-title":"27th Kerala Science Congress","author":"Abraham R","year":"2015","unstructured":"Abraham R, , Eswaran S. 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