{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:55:40Z","timestamp":1777877740804,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>\n                    Canine visceral leishmaniasis is a zoonotic disease caused by the protozoan\n                    <jats:italic toggle=\"yes\">Leishmania infantum<\/jats:italic>\n                    in Latin America. Visceral leishmaniasis (VL) diagnosis in Brazil includes two serological tests according to the Ministry of Health (MH) protocol. Sensitivity and specificity of diagnostic tests, as well as clinical signs of VL, are usually reported in disease-endemic areas; however, it is known that local epidemiological factors can influence these results. This study aimed to evaluate the clinical features, sensitivity, and specificity of TR-DPP\n                    <jats:sup>\u00ae<\/jats:sup>\n                    and EIE-LVC in naturally infected dogs in a region of sporadic VL transmission to humans in Brazil. A total of 288 dogs were clinically evaluated and serological and parasitological (lymph node aspirates) samples were collected for VL diagnosis. TR-DPP and EIE-LVC showed poor sensitivity (0.62 and 0.44, respectively) to detect infected animals, compared with the direct parasitological examination, which is considered a gold standard method. Thus, the protocol of MH presented low sensitivity (0.42) to estimate prevalence and control measures in this region. TR-DPP presented a high negative predictive value (0.89), resulting in its indication as a confirmatory test in sporadic transmission areas. Classical clinical signs of VL were not frequently observed; therefore, clinical scoring systems might not be useful in this region. Veterinarians of nonendemic areas should be alert for asymptomatic dogs, especially those presenting lymph adenomegaly.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2020.2701","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T23:32:57Z","timestamp":1621899177000},"page":"539-545","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Canine Visceral Leishmaniasis in an Area of Sporadic Transmission in Brazil"],"prefix":"10.1177","volume":"21","author":[{"given":"Leandro Machado","family":"Borges","sequence":"first","affiliation":[{"name":"Secretaria Municipal de Sa\u00fade de Camapu\u00e3\/MS - Setor de Zoonoses, Mato Grosso do Sul, Brasil."},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Parasit\u00e1rias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra Gutierrez de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Parasit\u00e1rias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."},{"name":"Laborat\u00f3rio de Parasitologia Humana, Instituto de Bioci\u00eancias, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nath\u00e1lia Lopes Fontoura","family":"Mateus","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Veterin\u00e1rias, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Everton Falc\u00e3o","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Parasit\u00e1rias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."},{"name":"Instituto Integrado de Sa\u00fade, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline Etelvina Casaril","family":"Arrua","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia Humana, Instituto de Bioci\u00eancias, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jucelei de Oliveira Moura","family":"Infran","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia Humana, Instituto de Bioci\u00eancias, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Bezerra","family":"Taketa","sequence":"additional","affiliation":[{"name":"Programa de Inicia\u00e7\u00e3o Cient\u00edfica (PIBIC), Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Eduardo de Oliveira","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Programa de Inicia\u00e7\u00e3o Cient\u00edfica (PIBIC), Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Eurico dos Santos","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia, Instituto de Bioci\u00eancias, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7238-2840","authenticated-orcid":false,"given":"Eliane Mattos","family":"Piranda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia Humana, Instituto de Bioci\u00eancias, Universidade Federal de Mato Grosso do Sul, Mato Grosso do Sul, Brasil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"e_1_3_4_2_1","unstructured":"Aguiar GM Medeiros WM. 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Topley & Wilson's Microbiology and Microbial Infections: Parasitology 10 ed. London: Hodder Arnold Press 2005:186\u2013199."},{"key":"e_1_3_4_10_1","first-page":"148","article-title":"New species of protozoa of the genus pathogenic leishmania to humans: Leishmania chagasi n.sp. (In Portuguese). Nota pr\u00e9via","author":"Cunha AM","year":"1937","unstructured":"Cunha AM, , Chagas E. New species of protozoa of the genus pathogenic leishmania to humans: Leishmania chagasi n.sp. (In Portuguese). Nota pr\u00e9via. 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