{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:09:21Z","timestamp":1792012161851,"version":"4.4.0"},"reference-count":41,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Culex pipiens<\/jats:italic>\n                    sensu lato has been documented as West Nile virus (WNV) vector in southeastern Romania. Bucharest, the densely populated capital city of Romania, and the surrounding Ilfov county are WNV hotspots. In this area, the morphologically indistinguishable biotypes of\n                    <jats:italic toggle=\"yes\">Cx. pipiens,<\/jats:italic>\n                    namely\n                    <jats:italic toggle=\"yes\">pipiens<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    , are usually differentiated by their behavioral and physiological traits. Their involvement in WNV transmission, as suggested by entomological investigations, was not previously documented for each biotype. We used a Real-Time PCR assay based on CQ11 microsatellite to identify the\n                    <jats:italic toggle=\"yes\">Cx. pipiens<\/jats:italic>\n                    biotypes and their hybrids collected in various habitats in the Bucharest metropolitan area. A sympatric distribution of both biotypes was observed, with a preference of green areas for\n                    <jats:italic toggle=\"yes\">pipiens<\/jats:italic>\n                    , and human settings and animal farmlands for\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    . In the latter habitats,\n                    <jats:italic toggle=\"yes\">pipiens<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    were found in mixed aboveground populations. A low number of hybrids was found suggesting existence of reproductive isolation. In subway tunnels\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    was dominant with a higher number of hybrids recorded than aboveground. Blood-engorged mosquitoes were identified to biotype and the blood meal source identified by DNA barcoding. Overall,\n                    <jats:italic toggle=\"yes\">Cx. pipiens<\/jats:italic>\n                    s.l. fed mainly on birds, commonly on house sparrows, collared doves, and blackbirds, which are potential WNV-amplifying hosts. The preference for avian hosts was expressed strongest by\n                    <jats:italic toggle=\"yes\">pipiens<\/jats:italic>\n                    biotype, while\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    was substantially less specific, feeding on avian and mammal hosts with similar frequency, with humans representing 20% of the hosts. Hybrids had a host choice closer to that of\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    . These findings highlight the role of\n                    <jats:italic toggle=\"yes\">pipiens<\/jats:italic>\n                    biotype as enzootic\/epizootic vector, and specifically show\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    as the bridge vector for WNV. The\n                    <jats:italic toggle=\"yes\">pipiens<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">molestus<\/jats:italic>\n                    biotypes show important differences in habitat preferences, including oviposition; these findings demonstrate that targeted mosquito control to limit WNV transmission may be possible.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2020.2739","type":"journal-article","created":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T10:27:53Z","timestamp":1624444073000},"page":"713-719","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":26,"title":["Characterization and Host-Feeding Patterns of\n                    <i>Culex pipiens<\/i>\n                    s.l. Taxa in a West Nile Virus-Endemic Area in Southeastern Romania"],"prefix":"10.1177","volume":"21","author":[{"given":"Georgiana Victori\u021ba","family":"Tiron","sequence":"first","affiliation":[{"name":"Cantacuzino National Medico-Military Institute for Research and Development, Bucharest, Romania."},{"name":"Faculty of Biology, University of Bucharest, Bucharest, Romania."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana Georgeta","family":"Stancu","sequence":"additional","affiliation":[{"name":"Cantacuzino National Medico-Military Institute for Research and Development, Bucharest, Romania."},{"name":"Faculty of Biology, University of Bucharest, Bucharest, Romania."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorin","family":"Dinu","sequence":"additional","affiliation":[{"name":"Cantacuzino National Medico-Military Institute for Research and Development, Bucharest, Romania."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian Liviu","family":"Prioteasa","sequence":"additional","affiliation":[{"name":"Cantacuzino National Medico-Military Institute for Research and Development, Bucharest, Romania."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"F\u0103lcu\u021b\u0103","sequence":"additional","affiliation":[{"name":"Cantacuzino National Medico-Military Institute for Research and Development, Bucharest, Romania."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia Svetlana","family":"Ceianu","sequence":"additional","affiliation":[{"name":"Cantacuzino National Medico-Military Institute for Research and Development, Bucharest, Romania."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ani Ioana","family":"Cotar","sequence":"additional","affiliation":[{"name":"Cantacuzino National Medico-Military Institute for Research and Development, Bucharest, Romania."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007092"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1725-9"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.2987\/8756-971X-28.4s.53"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-92874-4"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvec.12248"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6884120"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/088282401753266765"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-3145-5"},{"key":"e_1_3_4_10_1","first-page":"55","article-title":"[Role of small and minuscule pools and puddles of water in maintenance of urban Culex mosquitoes]","volume":"25","author":"Dancescu P","year":"1980","unstructured":"Dancescu P, , Dobrescu A, , Gafiteanu L. 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