{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:55:51Z","timestamp":1777877751578,"version":"3.51.4"},"reference-count":49,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:p>\n                    A clinical and entomological investigation was performed to identify flavivirus infections in humans and mosquitoes in impoverished areas of Guerrero, a coastal state in southwestern Mexico. A total of 639 patients with acute febrile illness and 830 resting female mosquitoes in low-income communities of Guerrero in 2019 were tested for evidence of flavivirus infection. Sera were collected from all patients and screened at a dilution of 1:20 by plaque reduction neutralization test (PRNT) using dengue virus (DENV)2. A total of 431 (67.4%) patients were seropositive. Sera from a subset of seropositive patients (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u2009263) were tested for flavivirus NS1 by enzyme-linked immunosorbent assay. Forty-eight (18.3%) sera contained viral antigen. All NS1-positive sera were titrated and further tested by PRNT using DENV-1 to -4, St. Louis encephalitis virus, West Nile virus, and Zika virus (ZIKV). Seven patients were seropositive for DENV-1, five patients were seropositive for DENV-2, one patient was seropositive for DENV-3, and two patients each were seropositive for DENV-4 and ZIKV. The remainder had secondary flavivirus infections or antibodies to an undetermined flavivirus. Comparative PRNTs were also performed on 60 randomly selected NS1-negative sera, identifying patients seropositive for DENV-2, DENV-3, and ZIKV. The entomological investigation yielded 736\n                    <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                    and 94\n                    <jats:italic toggle=\"yes\">Culex quinquefasciatus<\/jats:italic>\n                    that were sorted into 183 pools and 20 pools, respectively. Mosquitoes were assayed for flavivirus RNA by RT-PCR and Sanger sequencing. DENV-2 RNA was detected in three pools of\n                    <jats:italic toggle=\"yes\">A. aegypti<\/jats:italic>\n                    . In summary, we provide evidence for the concurrent circulation of all four DENVs and ZIKV in Guerrero, Mexico. The public health authorities reported no cases of DENV-3, DENV-4, and ZIKV in Guerrero in 2019 and thus, we provide evidence of under-reporting in the region.\n                  <\/jats:p>","DOI":"10.1089\/vbz.2020.2742","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T11:21:34Z","timestamp":1620127294000},"page":"458-465","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Co-Circulation of All Four Dengue Viruses and Zika Virus in Guerrero, Mexico, 2019"],"prefix":"10.1177","volume":"21","author":[{"given":"Daniel","family":"Nunez-Avellaneda","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandra","family":"Tangudu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Barrios-Palacios","sequence":"additional","affiliation":[{"name":"Experimental Pathology Section, National Institute of Medical Sciences and Nutrition Salvador Zubir\u00e1n, Ciudad de M\u00e9xico, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Machain-Williams","sequence":"additional","affiliation":[{"name":"Laboratorio de Arbovirologia, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucatan, Merida, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luz del Carmen","family":"Alarc\u00f3n-Romero","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Citopatolog\u00eda e Histoqu\u00edmica, Facultad de Ciencias Qu\u00edmico Biologicas, Universidad Aut\u00f3noma de Guerrero, Chilpancingo, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma Isabel","family":"Zubillaga-Guerrero","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Citopatolog\u00eda e Histoqu\u00edmica, Facultad de Ciencias Qu\u00edmico Biologicas, Universidad Aut\u00f3noma de Guerrero, Chilpancingo, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salatiel","family":"Nunez-Avellaneda","sequence":"additional","affiliation":[{"name":"Laboratorio de Analisis Cl\u00ednicos, Hospital General Adolfo Prieto, Taxco de Alarc\u00f3n, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren A.","family":"McKeen","sequence":"additional","affiliation":[{"name":"College of Liberal Arts and Sciences, Iowa State University, Ames, Iowa, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Israel","family":"Canche-Aguilar","sequence":"additional","affiliation":[{"name":"Departamento de Prevenci\u00f3n y Control de Enfermedades Transmitidas por Vector en el Estado de Guerrero, Chilpancingo, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Loaeza-D\u00edaz","sequence":"additional","affiliation":[{"name":"Laboratorio de Analisis Cl\u00ednicos, Hospital General Raymundo Abarca Alarc\u00f3n, Chilpancingo, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley J.","family":"Blitvich","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,5,27]]},"reference":[{"key":"e_1_3_4_2_1","unstructured":"Anonymous. 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