{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:56:01Z","timestamp":1777877761885,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Camel-to-human transmission of the Middle East Respiratory Syndrome coronavirus (MERS-CoV) was confirmed as a cause of primary infection in humans. There is a dearth of information regarding the behavior of the virus in camels and the mode of spread among them under natural conditions. The aim of this study was to monitor exposure of camels to the MERS-CoV under field conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>From January 1 to November 30, 2015, a secluded herd of 20 pregnant female camels and their neonate calves was established. Nasal and rectal swabs were collected from calves daily for 90 days after birth, then weekly until the end of the study. Nasal and rectal samples were collected from the dams at outset and then weekly until the end of the study. The samples were tested with rtRT-PCR to detect the MERS-CoV RNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>All purchased pregnant camels were MERS-CoV RNA negative at outset. Nineteen dams and 15 calves completed the study. Seven (46.7%) of the 15 calves developed a rise in rectal temperature (39\u201340\u00b0C), shivering, rhinitis, anorexia, and general weakness at a mean\u2009\u00b1\u2009standard deviation of 18.9\u2009\u00b1\u20094.9 days of age and their MERS-CoV RNA test was positive on the first day of illness. Three of the seven infected calves died 14\u2009\u00b1\u20099.1 days postonset of illness at age 17, 14, and 46 days, respectively. The remaining four infected calves fully recovered and they were MERS-CoV RNA positive for 17.5\u2009\u00b1\u20098.8 days. Four (21.1%) of the 19 dams had positive tests; three dams had no clinical signs, whereas the fourth dam exhibited signs not compatible with MERS-CoV infection and died three days after the positive test, 33 days after parturition. All MERS-CoV infections occurred within 22 days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This study has expanded our understanding of the MERS-CoV epidemiology among camels, which is an important step forward to device effective preventive measures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2021.0020","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T10:08:41Z","timestamp":1640599721000},"page":"994-1002","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Monitoring of the Middle East Respiratory Syndrome Coronavirus Activity in a Secluded Herd of Camels Kept Under Field Conditions"],"prefix":"10.1177","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2453-0623","authenticated-orcid":false,"given":"Tariq A.","family":"Madani","sequence":"first","affiliation":[{"name":"Department of Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia."},{"name":"Ministry of Health, Jeddah, Saudi Arabia."},{"name":"Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Fahad Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El-Tayb M.E.","family":"Abuelzein","sequence":"additional","affiliation":[{"name":"Ministry of Health, Jeddah, Saudi Arabia."},{"name":"Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Fahad Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."},{"name":"Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hashem S.","family":"Hussien","sequence":"additional","affiliation":[{"name":"Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Fahad Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6901-7500","authenticated-orcid":false,"given":"Mohammad A.","family":"Bashri","sequence":"additional","affiliation":[{"name":"Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Fahad Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Fahad Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."},{"name":"Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esam I.","family":"Azhar","sequence":"additional","affiliation":[{"name":"Ministry of Health, Jeddah, Saudi Arabia."},{"name":"Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Fahad Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."},{"name":"Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia."},{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Science, King Abdulaziz University, Jeddah, Saudi Arabia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,12,16]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000016652"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2012.141280"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu359"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2201.151340"},{"key":"e_1_3_5_6_1","unstructured":"Anon. 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