{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:56:25Z","timestamp":1777877785638,"version":"3.51.4"},"reference-count":45,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2022,4]]},"abstract":"\n Background:<\/jats:title>\n \n Klebsiella pneumoniae<\/jats:italic>\n has been associated with both nosocomial and community-acquired infections with mounting public health concern throughout the world. The purpose of this study was to investigate the burden of virulent extended-spectrum \u03b2-lactamase (ESBL)-producing\n K. pneumoniae<\/jats:italic>\n among diarrheic horses or those with respiratory illness to underscore the public health implication of such strains.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Rectal and nasal swabs were gathered from 100 diseased horses (50 diarrheic and 50 with respiratory illness). The collected swabs were processed for isolation of ESBL-producing\n K. pneumoniae<\/jats:italic>\n using a selective medium followed by phenotypic and molecular identification of the isolates. All ESBL-producing\n K. pneumoniae<\/jats:italic>\n strains were investigated for six virulence genes (type 3 fimbrial adhesin [\n mrk<\/jats:italic>\n D], enterobactin [\n ent<\/jats:italic>\n B], regulator of mucoid phenotype A [\n rmp<\/jats:italic>\n A],\n Klebsiella<\/jats:italic>\n ferric iron uptake [\n kfu<\/jats:italic>\n ], mucoviscosity-associated gene A [\n mag<\/jats:italic>\n A], and type 2 capsular polysaccharide [\n K2<\/jats:italic>\n ]).\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Of the 100 examined animals, ESBL-producing\n K. pneumoniae<\/jats:italic>\n was recovered from 13 (13%), with isolation rates in horses suffering from diarrhea and respiratory illness being 20% and 6%, respectively. Among the obtained isolates,\n bla<\/jats:italic>\n TEM and\n bla<\/jats:italic>\n SHV were found in all strains (100%) followed by\n bla<\/jats:italic>\n CTX-M in 92.3%, while none of the isolates had\n bla<\/jats:italic>\n OXA. In addition, 13 ESBL-producing\n K. pneumoniae<\/jats:italic>\n strains exhibited a multidrug resistance (MDR) pattern. Regarding the occurrence of virulence genes among the isolates,\n mrk<\/jats:italic>\n D (100%) and\n ent<\/jats:italic>\n B (100%) were the most predominant virulence genes followed by\n rmp<\/jats:italic>\n A (76.9%) and\n kfu<\/jats:italic>\n (46.2%). On the contrary,\n mag<\/jats:italic>\n A and\n K2<\/jats:italic>\n were negative in all ESBL-producing strains. Furthermore, this work provides four\n K. pneumoniae mrk<\/jats:italic>\n D partial sequences that displayed high genetic relatedness with those obtained from human to clarify the public health burden of such isolates.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n The occurrence of virulent ESBL-producing\n K. pneumoniae<\/jats:italic>\n among diseased horses highlights the potential role of this animal in the epidemiology of such virulent and antimicrobial-resistant strains, which may have great public health threat.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/vbz.2022.0004","type":"journal-article","created":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T11:21:11Z","timestamp":1649416871000},"page":"217-224","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["The Public Health Burden of Virulent Extended-Spectrum \u03b2-Lactamase-Producing\n Klebsiella pneumoniae<\/i>\n Strains Isolated from Diseased Horses"],"prefix":"10.1177","volume":"22","author":[{"given":"Ahmed","family":"Samir","sequence":"first","affiliation":[{"name":"Department of Microbiology and Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khaled A.","family":"Abdel-Moein","sequence":"additional","affiliation":[{"name":"Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hala M.","family":"Zaher","sequence":"additional","affiliation":[{"name":"Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,4,19]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-020-05157-4"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.738223"},{"key":"e_1_3_4_4_1","unstructured":"Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. 28th ed. CLSI supplement M100. Wayne PA: CLSI 2018."},{"key":"e_1_3_4_5_1","first-page":"137","article-title":"Urinary tract infection with Klebsiella pneumoniae in patients with chronic kidney disease","volume":"43","author":"Cristea OM","year":"2017","unstructured":"Cristea OM, , Avr\u0103mescu CS, , B\u0103l\u0103\u015foiu M, , Popescu FD, et al. Urinary tract infection with Klebsiella pneumoniae in patients with chronic kidney disease. Curr Health Sci J, 2017; 43:137\u2013148.","journal-title":"Curr Health Sci J"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.556654"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9070425"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1556\/030.66.2019.006"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku217"},{"key":"e_1_3_4_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030857"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01943-07"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-020-00875-w"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7279830"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2020.2847"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/s17061406"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00286-11"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-019-0660-x"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2006.21.5.816"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00052-11"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00483"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.02.035"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2013.10.002"},{"key":"e_1_3_4_23_1","first-page":"1057","article-title":"Occurrence of OXA-48 carbapenemase and other \u03b2-lactamase genes in ESBL-producing multidrug resistant Escherichia coli from dogs and cats in the United States, 2009-2013","volume":"7","author":"Liu X","year":"2016","unstructured":"Liu X, , Thungrat K, , Boothe DM. Occurrence of OXA-48 carbapenemase and other \u03b2-lactamase genes in ESBL-producing multidrug resistant Escherichia coli from dogs and cats in the United States, 2009-2013. Front Microbiol, 2016; 7:1057.","journal-title":"Front Microbiol"},{"key":"e_1_3_4_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks118"},{"key":"e_1_3_4_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_3_4_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01537-18"},{"key":"e_1_3_4_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux013"},{"key":"e_1_3_4_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00078-15"},{"key":"e_1_3_4_29_1","article-title":"Pyogenic liver abscess, bacteremia, and meningitis with hypermucoviscous Klebsiella pneumoniae: an unusual case report in a human T-cell lymphotropic virus positive patient of Caribbean origin in the United States","volume":"2013","author":"Patel G","year":"2013","unstructured":"Patel G, , Shah N, , Sharma R. Pyogenic liver abscess, bacteremia, and meningitis with hypermucoviscous Klebsiella pneumoniae: an unusual case report in a human T-cell lymphotropic virus positive patient of Caribbean origin in the United States. Case Rep Infect Dis, 2013; 2013:676340.","journal-title":"Case Rep Infect Dis"},{"key":"e_1_3_4_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_4_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.4.589"},{"key":"e_1_3_4_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9120852"},{"key":"e_1_3_4_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-187"},{"key":"e_1_3_4_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10020282"},{"key":"e_1_3_4_35_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22718"},{"key":"e_1_3_4_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mjafi.2016.05.002"},{"key":"e_1_3_4_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10030369"},{"key":"e_1_3_4_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01657"},{"key":"e_1_3_4_39_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.015198-0"},{"key":"e_1_3_4_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17176278"},{"key":"e_1_3_4_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-020-00875-w"},{"key":"e_1_3_4_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-015-2551-2"},{"key":"e_1_3_4_43_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S219155"},{"key":"e_1_3_4_44_1","doi-asserted-by":"publisher","DOI":"10.1086\/503420"},{"key":"e_1_3_4_45_1","article-title":"Occurrence of selected zoonotic fecal pathogens and first molecular identification of Hafnia paralvei in wild Taihangshan Macaques (Macaca mulatta tcheliensis) in China","volume":"2019","author":"Zhang Q","year":"2019","unstructured":"Zhang Q, , Han S, , Liu K, , Luo J, et al. Occurrence of selected zoonotic fecal pathogens and first molecular identification of Hafnia paralvei in wild Taihangshan Macaques (Macaca mulatta tcheliensis) in China. 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