{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:24:50Z","timestamp":1788143090656,"version":"build-2803163510"},"reference-count":41,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Leptospirosis is a contagious disease that affects domestic and wild animals as well as humans. It is caused by infection with some pathogenic species of the genus\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      . In Brazil, studies on leptospirosis in capybaras are scarce or nonexistent in some regions, such as the Federal District. The objective of this study was to analyze the presence of DNA of the agent and\/or anti-\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      spp. antibodies in capybaras.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      Blood samples were collected from 56 free-living capybaras captured in two different sites in the study region. The samples were submitted to hematology and clinical chemistry tests. To identify\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      positive samples, a conventional PCR (cPCR) and analysis of anti-\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      spp. antibodies by microscopic agglutination test (MAT) were used.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      No animal showed cPCR amplification of the Lip32 gene, but 41.1% (23\/56) of the animals had anti-\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      spp. antibodies on MAT. The serovars present were icterohaemorrhagiae (82.61%), copenhageni (65.22%), grippotyphosa (4.35%), and hardjo (4.35%). In the laboratorial tests, differences (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.05) were observed in the biochemical assays of alkaline phosphatase, creatinine, albumin, and globulin. Although these values differed significantly between groups, they all remained within reference range (excluding albumin), and thus there is not enough to infer that this alteration could be caused by\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      cPCR using whole blood samples to evaluate\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      spp. infection of free-living capybaras was not an efficient tool. The presence of\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      seroreactive capybaras shows that the bacteria are circulating in the urban environment of the Federal District.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2022.0034","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T07:46:07Z","timestamp":1677483967000},"page":"106-112","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Leptospira<\/i>\n                    spp. in Free-Ranging Capybaras (\n                    <i>Hydrochoerus hydrochaeris<\/i>\n                    ) from Midwestern Brazil"],"prefix":"10.1177","volume":"23","author":[{"given":"Thamiris F.","family":"da Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana P.N.","family":"de Quadros","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George M.S.","family":"do R\u00eago","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jussara T.","family":"de Medeiros","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz F.M.","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6427-878X","authenticated-orcid":false,"given":"Tai\u00e3 M.P.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa V.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Brasilia Zoo Foundation, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo S.R.","family":"de Mattos","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds A.","family":"Mathias","sequence":"additional","affiliation":[{"name":"School of Agricultural and Veterinary Studies, Jaboticabal, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giane R.","family":"Paludo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Agronomia e Medicina Veterin\u00e1ria\u2014FAV, Universidade de Bras\u00edlia\u2014UnB, Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,3,6]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.01.018"},{"key":"e_1_3_4_4_1","first-page":"464","volume-title":"Veterinary Hematology and Clinical Chemistry","author":"Allison RW","year":"2012","unstructured":"Allison RW. 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In: Capybara: Biology Use and Conservation of an Exceptional Neotropical Species. (Moreira JR Ferraz KMPMB Herrera EA et al. eds.) Springer: New York 2013; pp. 169\u2013184.","DOI":"10.1007\/978-1-4614-4000-0_9"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11252-020-00957-9"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/1980-549720210034"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2699.2006.01568.x"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11080710"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004403"},{"key":"e_1_3_4_17_1","first-page":"1973","volume-title":"Molecular Medical Microbiology","author":"Fraga TR","year":"2014","unstructured":"Fraga TR, , Carvalho E, , Isaac L, et al. Leptospira and Leptospirosis. In: Molecular Medical Microbiology. (Tang Y-W, , Sussman M, , Liu D, et al. eds.) 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Available from: https:\/\/g1.globo.com\/distrito-federal\/noticia\/secretaria-de-saude-apura-ataque-de-carrapatos-a-visitantes-do-zoo-do-df.ghtml [Last accessed: January 20 2023]."},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00058"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.5433\/1679-0359.2020v41n1p159"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.01.011"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007374"},{"key":"e_1_3_4_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vas.2019.100068"},{"key":"e_1_3_4_24_1","unstructured":"INMET\u2014Instituto Nacional de Meteorologia. Climatological norms (Normas Climatol\u00f3gicas). 2019. 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