{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:56:37Z","timestamp":1777877797909,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      The emergence or re-emergence of several orthobunyaviruses (order:\n                      <jats:italic toggle=\"yes\">Bunyavirales<\/jats:italic>\n                      ; family:\n                      <jats:italic toggle=\"yes\">Peribunyaviridae<\/jats:italic>\n                      ), including Cache Valley virus (CVV) and Oropouche virus, warrants the development and evaluation of candidate live-attenuated vaccines (LAVs). Ideally, these vaccines would elicit long-lasting immunity with one single immunization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>Since the deletion of two virulence factors, NSs and NSm, has been shown to attenuate the virulence phenotype of orthobunyaviruses, phleboviruses, and nairoviruses, genetic manipulation of the viral genome is considered an effective strategy for the rational design of candidate LAVs for bunyaviruses across multiple families. In addition, the deletion of Rift Valley fever virus NSs and NSm genes has been shown to reduce transmission by mosquitoes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      In this study, the ability of a CVV mutant lacking the NSs and NSm genes (2delCVV) to replicate in intrathoracically injected\n                      <jats:italic toggle=\"yes\">Aedes albopictus<\/jats:italic>\n                      was compared with the parental wild-type CVV (wtCVV) 6V633 strain. In contrast to the robust replication of wtCVV in injected mosquitoes, the multiplication kinetics of the 2delCVV mutant was reduced by more than a 100-fold.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>These results suggest that the deletion of NSm and NSs genes is a feasible approach to rationally design candidate orthobunyavirus LAVs that are highly attenuated in mosquitoes and, therefore, pose little risk of reversion to virulence and transmission.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2022.0053","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T10:40:59Z","timestamp":1668076859000},"page":"553-558","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Replication Kinetics of a Candidate Live-Attenuated Vaccine for Cache Valley Virus in\n                    <i>Aedes albopictus<\/i>"],"prefix":"10.1177","volume":"22","author":[{"given":"Victoria B.","family":"Ayers","sequence":"first","affiliation":[{"name":"Department of Diagnostic Medicine\/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA."},{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-Jang S.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine\/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA."},{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James I.","family":"Dunlop","sequence":"additional","affiliation":[{"name":"MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Kohl","sequence":"additional","affiliation":[{"name":"MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4707-726X","authenticated-orcid":false,"given":"Benjamin","family":"Brennan","sequence":"additional","affiliation":[{"name":"MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Higgs","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine\/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA."},{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana L.","family":"Vanlandingham","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine\/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA."},{"name":"Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-532"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2014.1669"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-3103-2"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3643-0"},{"key":"e_1_3_5_6_1","volume-title":"Viral Immunology","author":"Ayers VB","year":"2022","unstructured":"Ayers VB, , Huang YS, , Kohl A, et al. 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