{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:57:26Z","timestamp":1777877846054,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Brucellosis is the most important public health problem worldwide, and the annual incidence of the disease in humans is 2.1 million. The\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      genome is highly conserved, with over 90% similarity among species. The aim of this study was to perform species-level identification of\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      spp. strains isolated from humans diagnosed with brucellosis and to further investigate the phylogenetic relationships using multiple locus variable number of tandem repeats analysis (MLVA)-16 and 16S rRNA sequencing analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      spp. was isolated from the blood cultures of 54 patients who tested positive for brucellosis through serological examinations. Real-time PCR was used to identify the isolates in species, and the genus level of\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      was confirmed with 16S rRNA. All isolates were subjected to phylogenetic analysis using variable number of tandem repeat analysis with multiple loci.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Subsequent analysis via real-time PCR confirmed these isolates to be of the\n                      <jats:italic toggle=\"yes\">Brucella melitensis<\/jats:italic>\n                      species. The 16S rRNA sequence analysis showed 100% homogeneity among the isolates. MLVA revealed the formation of five different genotypic groups. While two groups were formed based on the 16S rRNA sequence analysis, five groups were formed in the MLVA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      The study concluded that 16S rRNA sequence analysis alone did not provide sufficient discrimination for phylogenetic analysis but served as a supportive method for identification. MLVA exhibited higher phylogenetic power. The widespread isolation of\n                      <jats:italic toggle=\"yes\">B. melitensis<\/jats:italic>\n                      from human brucellosis cases highlights the importance of controlling brucellosis in small ruminants to prevent human infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2023.0139","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T16:00:27Z","timestamp":1712937627000},"page":"416-423","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Phylogenetic Analysis of\n                    <i>Brucella melitensis<\/i>\n                    Strains Isolated from Humans Using 16S rRNA Sequencing and Multiple Locus Variable Number of Tandem Repeats Analysis-16"],"prefix":"10.1177","volume":"24","author":[{"given":"Berna","family":"Yanmaz","sequence":"first","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5665-6864","authenticated-orcid":false,"given":"Ediz Ka\u011fan","family":"\u00d6zgen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Atat\u00fcrk University, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orbay","family":"Say\u0131","sequence":"additional","affiliation":[{"name":"Veterinary Control Institute, Republic of T\u00fcrkiye Ministry of Agriculture and Forestry, \u0130zmir, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasemin","family":"Erdo\u011fan","sequence":"additional","affiliation":[{"name":"Veterinary Control Institute, Republic of T\u00fcrkiye Ministry of Agriculture and Forestry, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehtap H\u00fclya","family":"Aslan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Health Sciences University Erzurum Regional Education and Research Hospital, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibel","family":"\u0130ba Y\u0131lmaz","sequence":"additional","affiliation":[{"name":"Department of Infection Diseases and Clinical Microbiology, Health Sciences University Erzurum Regional Education and Research Hospital, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elif","family":"Karadeniz P\u00fct\u00fcr","sequence":"additional","affiliation":[{"name":"Veterinary Control Institute, Republic of T\u00fcrkiye Ministry of Agriculture and Forestry, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nebahat","family":"Polat","sequence":"additional","affiliation":[{"name":"Veterinary Control Institute, Republic of T\u00fcrkiye Ministry of Agriculture and Forestry, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"\u00d6zmen","sequence":"additional","affiliation":[{"name":"Veterinary Control Institute, Republic of T\u00fcrkiye Ministry of Agriculture and Forestry, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perihan","family":"\u015eerifo\u011flu Ba\u011fat\u0131r","sequence":"additional","affiliation":[{"name":"Veterinary Control Institute, Republic of T\u00fcrkiye Ministry of Agriculture and Forestry, Erzurum, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sedat","family":"Ild\u0131z","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences of Ankara University and General Directorate of Food and Control, Republic of T\u00fcrkiye Ministry of Agriculture and Forestry, Ankara, T\u00fcrkiye."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2022.109423"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.5158\/5\/gjvr.2022.1.0037"},{"issue":"3","key":"e_1_3_4_4_1","first-page":"108","article-title":"H. 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