{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:57:30Z","timestamp":1777877850859,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Dengue is a mosquito-borne tropical disease, caused by the Dengue virus (DENV). It has become a severe problem and is a rising threat to public health. In this study, we have evaluated commercial Merilisa i Dengue NS1 Antigen kit (Meril LifeSciences India Pvt. Ltd.) to detect recombinant dengue virus 2 NS1 antigen (rDNS1Ag) and secreted forms of NS1 antigen (sDNS1Ag).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      To determine the detection limit of the kit, 100 nanogram (ng) to 0.001 ng rDNS1Ag was tested. The sensitivity and specificity of the kit was determined using recombinant NS1 antigens of all serotypes of DENV and other flaviviruses. For testing sDNS1Ag, the culture supernatant of the Vero cell lines infected with DENV-2 was tested. Further, a spiking experiment was carried out to check the sensitivity of the kit to detect rDNS1Ag in the pools of\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      mosquitoes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>It was observed that the kit can detect the rDNS1Ag at 1 ng concentration. The kit was sensitive to detect NS1 antigen of DENV-1, DENV-2 and DENV-3 serotypes and specific for detection of only DNS1Ag as it did not cross-react with NS1 antigen of flaviviruses. The kit was sensitive to detect rDNS1Ag in the mosquito pools as well. In addition, the kit was able to detect the sDNS1Ag in Vero cell culture supernatant.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Overall, we observed that the Merilisa i Dengue NS1 Ag kit is sensitive and specific for the detection of DNS1Ag both in recombinant and secretory forms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2024.0009","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T22:00:23Z","timestamp":1723500023000},"page":"817-825","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Commercial Enzyme-Linked Immunosorbent Assay Kit Is Useful for Detection of Recombinant and Secretory Nonstructural-1 Protein Antigen of Dengue Virus"],"prefix":"10.1177","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4584-8139","authenticated-orcid":false,"given":"Philip Raj","family":"Abraham","sequence":"first","affiliation":[{"name":"ICMR-Vector Control Research Centre, Puducherry, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tharini","family":"Gopinath","sequence":"additional","affiliation":[{"name":"ICMR-Vector Control Research Centre, Puducherry, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akash","family":"Dhotre","sequence":"additional","affiliation":[{"name":"ICMR-Vector Control Research Centre, Puducherry, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashwani","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICMR-Vector Control Research Centre, Puducherry, India."},{"name":"Saveetha Institute of Medical and Technical Sciences, Saveetha University, Thandalam, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,12,2]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972-9062.328975"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-02965-x"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.11.020"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007420"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972-9062.159497"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15020572"},{"issue":"3","key":"e_1_3_5_8_1","first-page":"111","article-title":"Prevention and control of aedes aegypti-borne diseases: Lesson learned from past successes and failures","volume":"19","author":"Gubler DJ","year":"2011","unstructured":"Gubler DJ. Prevention and control of aedes aegypti-borne diseases: Lesson learned from past successes and failures. AsPac J Mol Biol Biotechnol, 2011; 19(3):111\u2013114.","journal-title":"AsPac J Mol Biol Biotechnol"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12080829"},{"issue":"7","key":"e_1_3_5_10_1","first-page":"551","article-title":"Emerging vector-borne diseases","volume":"94","author":"Huntington MK","year":"2016","unstructured":"Huntington MK, , Allison J, , Nair D. Emerging vector-borne diseases. Am Fam Physician, 2016; 94(7):551\u2013557.","journal-title":"Am Fam Physician"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_613_18"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.87"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.03.033"},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113411"},{"issue":"5","key":"e_1_3_5_15_1","first-page":"615","article-title":"Diagnostic methods for detection & isolation of dengue viruses from vector mosquitoes","volume":"123","author":"Philip Samuel P","year":"2006","unstructured":"Philip Samuel P, , Tyagi BK. Diagnostic methods for detection & isolation of dengue viruses from vector mosquitoes. Indian J Med Res, 2006; 123(5):615\u2013628.","journal-title":"Indian J Med Res"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-02215-9_4"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-155"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.12-0477"},{"key":"e_1_3_5_19_1","unstructured":"World Health Organization. Dengue guidelines for diagnosis treatment prevention and control: new edition. 2009; (WHO\/HTM\/NTD\/DEN\/2009.1)."},{"key":"e_1_3_5_20_1","unstructured":"World Health Organization. Dengue vaccines: WHO position paper\u2014September. 2018. Available from: www.who.int\/publications\/i\/item\/who-wer9335-457-476 [Last accessed: July4 2024]."},{"key":"e_1_3_5_21_1","volume-title":"Global Strategy for Dengue Prevention and Control 2012\u20132020","author":"World Health Organization","year":"2012","unstructured":"World Health Organization. Global Strategy for Dengue Prevention and Control 2012\u20132020. World Health Organization: Geneva; 2012."},{"key":"e_1_3_5_22_1","unstructured":"World Health Organization. Vector-borne diseases. 2020 Available from: www.who.int\/news-room\/fact-sheets\/detail\/vector-borne-diseases [Last accessed: January16 2024]."},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-322"}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/vbz.2024.0009","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2024.0009","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2024.0009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:03:58Z","timestamp":1777590238000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/vbz.2024.0009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":22,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1089\/vbz.2024.0009"],"URL":"https:\/\/doi.org\/10.1089\/vbz.2024.0009","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12]]}}}