{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:39:00Z","timestamp":1789133940263,"version":"build-2803163510"},"reference-count":45,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Most of the rickettsioses are transmitted by ticks, and often overlooked by the medical profession, but are clinically important as they cause major human diseases. Recent studies have shown the existence of some rickettsial species in Iran, but very little information is available about the status of rickettsial epidemiology and ecology. This study investigated the presence of\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      spp. in ticks and ruminants in western of Iran by molecular methods.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      250 blood samples were collected from sheep and goats, as well as 244 ticks were collected opportunistically from ruminants in the Kurdistan province. The collected samples were tested using a real-time quantitative PCR (qPCR) assay targeting the\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      <jats:italic toggle=\"yes\">16SrRNA<\/jats:italic>\n                      gene.\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      spp. positive by the qPCR were further amplified by conventional PCR of the\n                      <jats:italic toggle=\"yes\">gltA<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">OmpA<\/jats:italic>\n                      genes. These ampliqons were further analyzed by sequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The ticks species collected in this study included\n                      <jats:italic toggle=\"yes\">Rhipicephalus sanguineus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Rh. turanicus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Haemaphysalis concinna<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Dermacentor marginatus<\/jats:italic>\n                      . In total, DNA of\n                      <jats:italic toggle=\"yes\">Rickettsia<\/jats:italic>\n                      spp. was detected in 131 collected ticks (53.7%). Of the positives,\n                      <jats:italic toggle=\"yes\">Rickettsia slovaca<\/jats:italic>\n                      (59.2%) and\n                      <jats:italic toggle=\"yes\">Ri. hoogstraalii<\/jats:italic>\n                      (16.3%) were the most common species identified followed by\n                      <jats:italic toggle=\"yes\">Ri. raoultii<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Ri. massiliae<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Ri. sibirica<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Ri. conorii<\/jats:italic>\n                      subsp.\n                      <jats:italic toggle=\"yes\">israelensis<\/jats:italic>\n                      . In contrast, there were no positives observed in the blood samples collected from ruminants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The results indicate the presence of rickettsial species in ticks. The detection of these pathogens is significant because they cause clinical disease in humans. The results support the notion that the Iranian public health system needs to be more aware of these diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2024.0014","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T10:00:40Z","timestamp":1718013640000},"page":"730-737","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of Various Rickettsial Species in Ticks Collected from Small Ruminants in Western Iran"],"prefix":"10.1177","volume":"24","author":[{"given":"Meysam","family":"Moravedji","sequence":"first","affiliation":[{"name":"Department of Veterinary, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina","family":"Latifian","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Plague, Tularemia and Q fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran."},{"name":"Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Rahravani","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan","family":"Mostafavi","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Plague, Tularemia and Q fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran."},{"name":"Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Seyfi","sequence":"additional","affiliation":[{"name":"Department of Veterinary, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrdad","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Veterinary, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Mahdi","family":"Mozoun","sequence":"additional","affiliation":[{"name":"Department of Veterinary, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Stenos","sequence":"additional","affiliation":[{"name":"Australian Rickettsial Reference Laboratory, University Hospital Geelong, Geelong 3220, VIC, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saber","family":"Esmaeili","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Plague, Tularemia and Q fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar Ahang, Hamadan, Iran."},{"name":"Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,11,6]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf00140130"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01728-17"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2022.101798"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.2160\/8\/EJVS.2019.19553.1127"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2237-y"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11090973"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-59488-0"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25194431"},{"key":"e_1_3_5_10_1","first-page":"142","article-title":"Two strains of Rickettsiae of rocky mountain SFG recovered from D. marginatus ticks in Czechoslovakia. 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