{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T20:27:52Z","timestamp":1778790472636,"version":"3.51.4"},"reference-count":9,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>Widespread transmission of Japanese encephalitis virus (JEV) genotype four (GIV) occurred across mainland Australia in 2022. This resulted in forty-five human cases, including seven deaths, and the identification of JEV infection in over 80 commercial piggeries.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      We collected mosquitoes which were trapped using CO\n                      <jats:sub>2<\/jats:sub>\n                      -baited light traps deployed near piggeries reporting disease or in regions linked to human cases in the Wide Bay region in the state of Queensland. Mosquitoes from four traps yielded JEV RNA by real-time RT-PCR. Pools containing RNA positive mosquitoes were inoculated onto mosquito cell monolayers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion:<\/jats:title>\n                    <jats:p>A single isolate of JEV was obtained from a pool of mixed mosquito species. Near whole genome sequencing and phylogenetic analysis of the JEV isolate demonstrated its high genomic relatedness with JEV GIV pig sequences sampled from Queensland and the state of New South Wales in 2022.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>We report the first isolation of JEV GIV from mosquitoes collected in Australia. With only a few JEV GIV isolates available globally, the isolate we report will be essential for future research of JEV host interactions, evolution and disease markers, and development of effective therapies, vaccines, diagnostic assays, and mosquito control strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2024.0017","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T15:37:56Z","timestamp":1713195476000},"page":"439-442","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["First Isolation of Japanese Encephalitis Virus Genotype IV from Mosquitoes in Australia"],"prefix":"10.1177","volume":"24","author":[{"given":"Alyssa T.","family":"Pyke","sequence":"first","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Burtonclay","sequence":"additional","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirdesh","family":"Poudel","sequence":"additional","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne","family":"Ingall","sequence":"additional","affiliation":[{"name":"Wide Bay Public Health Unit, Wide Bay Hospital and Health Service, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelima","family":"Nair","sequence":"additional","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Hall-Mendelin","sequence":"additional","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott B.","family":"Craig","sequence":"additional","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig","family":"Smith","sequence":"additional","affiliation":[{"name":"Biosecurity Queensland, Department of Agriculture and Fisheries, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan M.","family":"Darbro","sequence":"additional","affiliation":[{"name":"Metro North Public Health Unit, Queensland Health, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassie C.","family":"Jansen","sequence":"additional","affiliation":[{"name":"Communicable Diseases Branch and Health Protection and Regulation Branch, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew F.","family":"van den Hurk","sequence":"additional","affiliation":[{"name":"Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"e_1_3_5_2_1","unstructured":"Australian Government Department of Agriculture Fisheries and Forestry (DAFF). Japanese Encephalitis Virus. Available from: https:\/\/www.agriculture.gov.au\/biosecurity-trade\/pests-diseases-weeds\/animal\/japanese-encephalitis (Last accessed: December 5 2023]."},{"key":"e_1_3_5_3_1","unstructured":"Australian Government Department of Health and Aged Care (DoHAC). Statement on the End of Japanese Encephalitis Virus Emergency Response. Available from: https:\/\/www.health.gov.au\/news\/statement-on-the-end-of-japanese-encephalitis-virus-emergency-response [Last accessed: December 5 2023]."},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.10.085233"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2512.181810"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14112480"},{"key":"e_1_3_5_7_1","unstructured":"Marks EN. An Atlas of Common Queensland Mosquitoes. Queensland Institute of Medical Research: Brisbane Australia; 1982."},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed5030133"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2207004"},{"key":"e_1_3_5_10_1","unstructured":"World Organisation for Animal Health (WOAH). OIE Immediate Notification\u2014Japanese Encephalitis Australia; WOAH: Paris France; 2022. Available from: https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2022-DON365 [Last accessed: December5 2023]."}],"container-title":["Vector-Borne and Zoonotic Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/vbz.2024.0017","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2024.0017","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/vbz.2024.0017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T23:04:00Z","timestamp":1777590240000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/vbz.2024.0017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":9,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1089\/vbz.2024.0017"],"URL":"https:\/\/doi.org\/10.1089\/vbz.2024.0017","relation":{},"ISSN":["1530-3667","1557-7759"],"issn-type":[{"value":"1530-3667","type":"print"},{"value":"1557-7759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]}}}