{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T12:23:29Z","timestamp":1780662209413,"version":"3.54.1"},"reference-count":9,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Vector-Borne and Zoonotic Diseases"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Borrelia burgdorferi sensu stricto<\/jats:italic>\n                      is the causative agent of Lyme disease (LD). Possible early symptoms include flu-like symptoms and erythema migrans and later, the risk of disruption of the nervous system, joints, and heart. A two-tiered testing method is employed for serological diagnostics. The Public Health Agency of Canada guidelines recommend that samples tested negative on first-tiered test need not be confirmed by second-tiered test. Due to the challenging nature of diagnosis leading to misconceptions among physicians about false negatives, confirmatory testing is requested despite the initial negative result.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Hundred screen-negative Lyme patient samples from 2007 to 2016 were tested by Western blot (WB) second-tiered confirmatory test upon physician\u2019s request in British Columbia to study the first-tiered screening test sufficiency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Those negative for first-tiered enzyme-linked immunosorbent assay were also negative by WB.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Results demonstrate that confirmatory testing is not necessary on screen-negative samples. Hence, first-tiered test is sufficient to rule out LD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/vbz.2024.0042","type":"journal-article","created":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T13:18:43Z","timestamp":1721740723000},"page":"835-837","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Lyme Disease Confirmatory Western Blot Is Redundant for Screen Negative Samples in Low Endemic Areas, British Columbia, Canada"],"prefix":"10.1177","volume":"24","author":[{"given":"Emily","family":"Kon","sequence":"first","affiliation":[{"name":"University of British Columbia, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hansi","family":"Adikari","sequence":"additional","affiliation":[{"name":"British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Simpson","sequence":"additional","affiliation":[{"name":"British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quantine","family":"Wong","sequence":"additional","affiliation":[{"name":"British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Laley","sequence":"additional","affiliation":[{"name":"British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navdeep","family":"Chahil","sequence":"additional","affiliation":[{"name":"British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Morshed","sequence":"additional","affiliation":[{"name":"University of British Columbia, Vancouver, Canada."},{"name":"British Columbia Centre for Disease Control Public Health Laboratory, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,12,2]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.18.3.484-509.2005"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00200-08"},{"key":"e_1_3_5_4_1","unstructured":"Canada PHA of Government of Canada (Gouvernement du Canada). 2023. 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