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To incorporate the diagnosis of this viral disease in a multiple-diagnostic assay, we first analyzed by immunoinformatics the HCV subtype 1a polyprotein (specifically Core, E2, NS3, NS5A proteins) to select antigenic peptides to be tested initially by the Pepscan technique. Next, we performed the immunodiagnosis of HCV infection, using the Multiple Antigen Blot Assay (MABA). In 22 patients' sera included in this study, a 20-mer linear peptide belonging to the\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    -terminus of the worldwide conserved Core protein showed 100% sensitivity and specificity; other sequences showed different levels of antibody recognition. The use of MABA in combination with synthetic peptides as a source of multiple, specific, and nonexpensive antigens for other infectious diseases could represent a rapid, integrated, and inexpensive diagnostic methodology.\n                  <\/jats:p>","DOI":"10.1089\/vim.2018.0023","type":"journal-article","created":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T16:39:46Z","timestamp":1537979986000},"page":"568-574","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Use of Synthetic Peptides and Multiple Antigen Blot Assay in the Immunodiagnosis of Hepatitis C Virus Infection"],"prefix":"10.1177","volume":"31","author":[{"given":"Adriana","family":"Gauna","sequence":"first","affiliation":[{"name":"Pontificia Universidad Cat\u00f3lica de Valpara\u00edso\/Universidad T\u00e9cnica Federico Santa Mar\u00eda","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Losada","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical","place":["Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical","place":["Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyan","family":"Toledo","sequence":"additional","affiliation":[{"name":"Universidad Central de Venezuela","place":["Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Berm\u00fadez","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical","place":["Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierina","family":"D'Angelo","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Higiene \u201cRafael Rangel","place":["Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doneyla","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Higiene \u201cRafael Rangel","place":["Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar","family":"Noya","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical","place":["Venezuela"]},{"name":"Instituto de Altos Estudios \u201cDr. Arnoldo Gabald\u00f3n\u201d Instituto Nacional de Higiene-Ministerio del Poder Popular para la Salud","place":["Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,10,15]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-010-0171-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/psc.2836"},{"key":"e_1_3_3_4_2","first-page":"250","volume-title":"Reports on the Sixth International Congress in Immunology","author":"Boger J","year":"1986","unstructured":"Boger J, , Emini EA, , and Schmidt A. 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