{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T10:55:05Z","timestamp":1773312905934,"version":"3.50.1"},"reference-count":57,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Viral Immunology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>\n                    Porcine reproductive and respiratory syndrome virus (PRRSV) still poses a threat to the swine industry worldwide. Currently, commercial vaccines against PRRSV, which consist of modified live or inactivated virus, reduce symptoms and viremia in immunized pigs, but efficacy against heterologous strains is variable. This has led to the development of subunit vaccines that contain viral antigens that show the highest variability. In this work, a chimeric protein comprising short amino acid sequences from glycoprotein 3 (GP3), glycoprotein 4 (GP4), glycoprotein 5 (GP5), and M (matrix protein) proteins of PRRSV was designed and expressed in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    . This protein, designated as PRRSVchim, was purified by immobilized metal affinity chromatography and evaluated. PRRSVchim was identified by immunoglobulin G (IgG) presence in serum samples from PRRSV-positive pigs. Also, the protein probed to be antigenic in immunized mice and piglets and provided some degree of protection against challenge with a PRRSV field isolate. These results show the potential of PRRSVchim protein for both PRRSV diagnostic and immunoprophylaxis.\n                  <\/jats:p>","DOI":"10.1089\/vim.2019.0047","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T14:04:47Z","timestamp":1571839487000},"page":"370-382","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["An\n                    <i>Escherichia coli<\/i>\n                    -Expressed Porcine Reproductive and Respiratory Syndrome Virus Chimeric Protein Induces a Specific Immunoglobulin G Response in Immunized Piglets"],"prefix":"10.1177","volume":"32","author":[{"given":"Darwin","family":"Elizondo-Quiroga","sequence":"first","affiliation":[{"name":"Unidad de Biotecnolog\u00eda M\u00e9dica y Farmac\u00e9utica, Centro de Investigaci\u00f3n y Asistencia en Tecnolog\u00eda y Dise\u00f1o del Estado de Jalisco, Guadalajara, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Zapata-Cuellar","sequence":"additional","affiliation":[{"name":"Unidad de Biotecnolog\u00eda M\u00e9dica y Farmac\u00e9utica, Centro de Investigaci\u00f3n y Asistencia en Tecnolog\u00eda y Dise\u00f1o del Estado de Jalisco, Guadalajara, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Alberto","family":"Uribe-Flores","sequence":"additional","affiliation":[{"name":"Unidad de Biotecnolog\u00eda M\u00e9dica y Farmac\u00e9utica, Centro de Investigaci\u00f3n y Asistencia en Tecnolog\u00eda y Dise\u00f1o del Estado de Jalisco, Guadalajara, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Gaona-Bernal","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Patolog\u00eda, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanya Amanda","family":"Camacho-Villegas","sequence":"additional","affiliation":[{"name":"CONACYT\u2014Unidad de Biotecnolog\u00eda M\u00e9dica y Farmac\u00e9utica, Centro de Investigaci\u00f3n y Asistencia en Tecnolog\u00eda y Dise\u00f1o del Estado de Jalisco, Guadalajara, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Alberto","family":"Manuel-Cabrera","sequence":"additional","affiliation":[{"name":"Receptor Biotec S.A. de C.V., Lagos de Moreno, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Elena","family":"Trujillo-Ortega","sequence":"additional","affiliation":[{"name":"Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Ram\u00edrez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco Antonio","family":"Herradora-Lozano","sequence":"additional","affiliation":[{"name":"Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda del C\u00e1rmen","family":"Mercado-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abel","family":"Guti\u00e9rrez Ortega","sequence":"additional","affiliation":[{"name":"Unidad de Biotecnolog\u00eda M\u00e9dica y Farmac\u00e9utica, Centro de Investigaci\u00f3n y Asistencia en Tecnolog\u00eda y Dise\u00f1o del Estado de Jalisco, Guadalajara, M\u00e9xico."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,11,7]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-017-2836-z"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091918"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12015"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci6010026"},{"key":"e_1_3_3_6_2","first-page":"26","article-title":"The structural biology of PRRSV","volume":"6","author":"Dokland T","year":"2010","unstructured":"Dokland T. 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