{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:04:36Z","timestamp":1780927476793,"version":"3.54.1"},"reference-count":40,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Viral Immunology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:p>Pneumonia is a pulmonary disease among children. Evodiamine, a traditional Chinese medicine, is known for anti-inflammatory effect. This study aimed to investigate the impact of evodiamine on severe pneumonia-like cells and the underlying mechanism involved. H5N1 and pneumoniae D39 was used to induce severe pneumonia-like conditions in BEAS-2B cells. The cell viability in BEAS-2B cells after treatments with 0, 20, 40, 60, 80, and 100\u2009\u03bcM evodiamine was examined using MTT assays. The protein concentrations of inflammatory cytokines tumor necrosis factor (TNF)-\u03b1, interleukin (IL)-6 and IL-1\u03b2, and Toll-like receptors (TLRs) were measured by enzyme-linked immunosorbent assay methods and the protein and mRNA changes in C\/EBP\u03b2\/CREB were measured using Real Time-quantitative polymerase chain reaction and Western blot methods. Our results revealed that Evodiamine significantly decreased TNF-\u03b1, IL-6, and IL-1\u03b2 in BEAS-2B cells. Moreover, evodiamine markedly reduced TLR2,3,4 protein expression and the phosphorylated protein of C\/EBP\u03b2 and CREB. Besides, evodiamine combined with clindamycin exerted more significant effects than clindamycin alone. Taken together, our results demonstrated that evodiamine enhanced the anti-inflammation effect of clindamycin in the BEAS-2B cells infected with H5N1 and pneumoniae D39 through CREB-C\/EBP\u03b2 signaling pathway.<\/jats:p>","DOI":"10.1089\/vim.2020.0319","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T16:30:38Z","timestamp":1620145838000},"page":"410-415","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Evodiamine Enhanced the Anti-Inflammation Effect of Clindamycin in the BEAS-2B Cells Infected with H5N1 and Pneumoniae D39 Through CREB-C\/EBP\u03b2 Signaling Pathway"],"prefix":"10.1177","volume":"34","author":[{"given":"Xiaqing","family":"Liu","sequence":"first","affiliation":[{"name":"Children's Respiratory Department, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1865-8500","authenticated-orcid":false,"given":"Xiaofang","family":"Peng","sequence":"additional","affiliation":[{"name":"Cell and Molecular Diagnosis Center, Sun Yat Sen Memorial Hospital, Sun Yat Sen University, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengfang","family":"Lin","sequence":"additional","affiliation":[{"name":"Center Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,8,13]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.336.6088.1521"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032414-112240"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nep238"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042706"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.03.014"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.041"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/139.6.681"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161226093"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173569"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R114.619957"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3310\/hta20420"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.4149\/av_2009_04_225"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2016.11.012"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2018.09.012"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep03799"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-74604-w"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.7.5075"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/596051"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)00153-5"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12408"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccm.2016.11.005"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2018.01.011"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2017.01.039"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e31819940cb"},{"key":"e_1_3_4_26_2","first-page":"27","article-title":"Inducible clindamycin resistance in \u03b2-hemolytic streptococci and Streptococcus pneumoniae","volume":"15","author":"Megged O","year":"2013","unstructured":"Megged O, , Assous M, , Weinberg G, et al. 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