{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T19:11:55Z","timestamp":1784920315542,"version":"3.55.0"},"reference-count":41,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Women's Health Reports"],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Most women who are treated at\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      fertilization (IVF) clinics have trouble conceiving due to ovarian failure (OF), which seems to be associated to short telomeres and reduced or absent telomerase activity in their granulosa cells. Indeed, telomere pathways are involved in organ dysfunction. However, sexual steroids can stimulate the expression of the telomerase gene and have been successfully used to prevent telomere attrition. Thus, a strategy to improve IVF outcomes in women with OF could be telomerase reactivation using sexual steroids.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>We conducted a double-blind, placebo-controlled study. Patients with diminished ovarian reserve were randomized to Danazol or placebo for 3 months. We included patients with normal ovarian reserve in the study as untreated controls. Patients and controls underwent several ovarian stimulations (OSs). Telomere and IVF parameters were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      We found that the mean telomere length in blood and the percentage of short and long telomeres were similar throughout the 3 months of treatment with Danazol. Remarkably, while the number of cells with one telomeric repeat-containing RNA (TERRA) focus decreased (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009=\u20090.04) after the first month of Danazol treatment, the number of cells with 2 to 4 TERRA foci increased (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009=\u20090.02). Regarding fertility, no differences were found in the antral follicle count. Interestingly, in OS performed after the trial, all Danazol-treated patients had a better MII oocyte rate compared to OS performed before the pilot study.\n                    <\/jats:p>\n                    <jats:p>EudraCT number: 2018-004400-19.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Danazol treatment seemed to affect telomere maintenance, since both the number of TERRA foci and the ratio of MII oocytes changed. However, further research is needed to confirm these results.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/whr.2023.0013","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T15:53:11Z","timestamp":1688572391000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["A Randomized Controlled Intervention Trial with Danazol to Improve Telomeric and Fertility Parameters in Women with Diminished Ovarian Reserve: A Pilot Study"],"prefix":"10.1177","volume":"4","author":[{"given":"Isabel","family":"C\u00f3rdova-Oriz","sequence":"first","affiliation":[{"name":"The Health Research Institute La Fe (IIS La Fe), IVI Foundation, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela","family":"Kohls","sequence":"additional","affiliation":[{"name":"IVIRMA Madrid, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Iglesias","sequence":"additional","affiliation":[{"name":"IVIRMA Madrid, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba M.","family":"Polonio","sequence":"additional","affiliation":[{"name":"The Health Research Institute La Fe (IIS La Fe), IVI Foundation, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda","family":"Chico-Sordo","sequence":"additional","affiliation":[{"name":"The Health Research Institute La Fe (IIS La Fe), IVI Foundation, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Toribio","sequence":"additional","affiliation":[{"name":"IVIRMA Madrid, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos","family":"Meseguer","sequence":"additional","affiliation":[{"name":"The Health Research Institute La Fe (IIS La Fe), IVI Foundation, Valencia, Spain."},{"name":"Laboratory of In Vitro Fertilization, IVIRMA Valencia, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5361-3877","authenticated-orcid":false,"given":"Elisa","family":"Varela","sequence":"additional","affiliation":[{"name":"The Health Research Institute La Fe (IIS La Fe), IVI Foundation, Valencia, Spain."},{"name":"Department of Medical Specialties and Public Health, Rey Juan Carlos University, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Pellicer","sequence":"additional","affiliation":[{"name":"The Health Research Institute La Fe (IIS La Fe), IVI Foundation, Valencia, Spain."},{"name":"School of Medicine, Department of Obstetrics and Gynecology, University of Valencia, Valencia, Spain."},{"name":"IVIRMA Rome, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Garc\u00eda-Velasco","sequence":"additional","affiliation":[{"name":"The Health Research Institute La Fe (IIS La Fe), IVI Foundation, Valencia, Spain."},{"name":"IVIRMA Madrid, Madrid, Spain."},{"name":"Department of Medical Specialties and Public Health, Rey Juan Carlos University, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,7,4]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2021.111541"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/GCO.0000000000000779"},{"issue":"4","key":"e_1_3_6_4_2","first-page":"561","article-title":"Impact of ovarian aging in reproduction: From telomeres and mice models to ovarian rejuvenation","volume":"93","author":"Polonio AM","year":"2020","unstructured":"Polonio AM, , Chico-Sordo L, , Cordova-Oriz I, et al. 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