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In this study, the goal was to establish protocols for sperm cryopreservation and artificial insemination to produce live young in\n                    <jats:italic toggle=\"yes\">X. variatus,<\/jats:italic>\n                    and to identify needs for repository development. The objectives were to: 1) collect basic biological characteristics of males; 2) cryopreserve sperm from\n                    <jats:italic toggle=\"yes\">X. variatus<\/jats:italic>\n                    , 3) harvest live young from cryopreserved sperm, and 4) discuss the requirements for establishment of sperm repositories. The 35 males used in this study had a body weight of 0.298\u00b10.096\u2009g (mean\u00b1SD), body length of 2.5\u00b10.2\u2009cm, and testis weight of 6.4\u00b13.4\u2009mg. The sperm production per gram of testis was 2.33\u00b11.32\u00d710\n                    <jats:sup>9<\/jats:sup>\n                    cells. After freezing, the post-thaw motility decreased significantly to 37%\u00b117% (ranging from 5% to 70%) (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    =0.000) from 57%\u00b114% (40%\u201380%) of fresh sperm (N=20). Artificial insemination of post-thaw sperm produced confirmed offspring from females of\n                    <jats:italic toggle=\"yes\">X. hellerii<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">X. variatus<\/jats:italic>\n                    . This research, taken together with previous studies, provides a foundation for development of strategies for sperm repositories of\n                    <jats:italic toggle=\"yes\">Xiphophorus<\/jats:italic>\n                    fishes. This includes: 1) the need for breeding strategies for regeneration of target populations, 2) identification of minimum fertilization capacity of frozen samples, 3) identification of fish numbers necessary for sampling and their genetic relationships, 4) selection of packaging containers for labeling and biosecurity, 5) assurance of quality control and standardization of procedures, 6) information systems that can manage the data associated with cryopreserved samples, including the genetic data, 7) biological data of sampled fish, 8) inventory data associated with frozen samples, and 9) data linking germplasm samples with other related materials such as body tissues or cells saved for DNA and RNA analyses.\n                  <\/jats:p>","DOI":"10.1089\/zeb.2012.0737","type":"journal-article","created":{"date-parts":[[2012,8,27]],"date-time":"2012-08-27T11:53:36Z","timestamp":1346068416000},"page":"126-134","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Sperm Cryopreservation in Live-Bearing\n                    <i>Xiphophorus<\/i>\n                    Fishes: Offspring Production from\n                    <i>Xiphophorus variatus<\/i>\n                    and Strategies for Establishment of Sperm Repositories"],"prefix":"10.1177","volume":"9","author":[{"given":"Huiping","family":"Yang","sequence":"first","affiliation":[{"name":"Louisiana State University Agricultural Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Cuevas-Uribe","sequence":"additional","affiliation":[{"name":"Louisiana State University Agricultural Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markita G.","family":"Savage","sequence":"additional","affiliation":[{"name":"Texas State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald B.","family":"Walter","sequence":"additional","affiliation":[{"name":"Texas State University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terrence R.","family":"Tiersch","sequence":"additional","affiliation":[{"name":"Louisiana State University Agricultural Center"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,8,27]]},"reference":[{"key":"e_1_3_3_2_2","volume-title":"Cryopreservation in Aquatic Species","author":"Tiersch TR","year":"2011","unstructured":"Tiersch TR, , Green CC. 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