{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:22:46Z","timestamp":1790709766381,"version":"4.1.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Zebrafish"],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mammalian models of cardiac disease have provided unique and important insights into human disease but have become increasingly challenging to produce. The zebrafish could provide inexpensive high-throughput models of cardiac injury and repair. We used a highly targeted laser, synchronized to fire at specific phases of the cardiac cycle, to induce regional injury to the ventricle, atrioventricular (AV) cushion, and bulbus arteriosus (BA). We assessed the impact of laser injury on hearts of zebrafish early larvae at 72\u2009h postfertilization, to different regions, recording the effects on ejection fraction (EF), heart rate (HR), and blood flow at 2 and 24\u2009h postinjury (hpi). Laser injury to the apex, midzone, and outflow regions of the ventricle resulted in reductions of the ventricle EF at 2 hpi with full recovery of function by 24 hpi. Laser injury to the ventricle, close to the AV cushion, was more likely to cause bradycardia and atrial\u2013ventricular dysfunction, suggestive of an electrical conduction block. At 2 hpi, direct injury to the AV cushion resulted in marked regurgitation of blood from the ventricle to the atrium. Laser injury to the BA caused temporary outflow tract obstruction with cessation of ventricle contraction and circulation. Despite such damage, 80% of embryos showed complete recovery of the HR and function within 24\u2009h of laser injury. Precision laser injury to key structures in the zebrafish developing heart provides a range of potentially useful models of hemodynamic overload, injury, and repair.<\/jats:p>","DOI":"10.1089\/zeb.2014.1027","type":"journal-article","created":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T15:48:52Z","timestamp":1412178532000},"page":"536-541","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Targeted Laser Ablation of the Zebrafish Larval Heart Induces Models of Heart Block, Valvular Regurgitation, and Outflow Tract Obstruction"],"prefix":"10.1177","volume":"11","author":[{"given":"Gianfranco","family":"Matrone","sequence":"first","affiliation":[{"name":"The University of Edinburgh","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Maqsood","sequence":"additional","affiliation":[{"name":"The University of Edinburgh","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Taylor","sequence":"additional","affiliation":[{"name":"University of Glasgow","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Mullins","sequence":"additional","affiliation":[{"name":"The University of Edinburgh","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl S.","family":"Tucker","sequence":"additional","affiliation":[{"name":"The University of Edinburgh","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin A.","family":"Denvir","sequence":"additional","affiliation":[{"name":"The University of Edinburgh","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,10,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-110711-155646"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12111"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20206"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050053"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0185(20001001)260:2<148::AID-AR50>3.0.CO;2-X"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/1050-1738(94)90036-1"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.21037"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780199638086.001.0001"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.53.030191.000543"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04520.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.143990"},{"key":"e_1_3_3_13_2","article-title":"Laser ablation of the zebrafish pronephros to study renal epithelial regeneration","volume":"54","author":"Johnson CS","year":"2011","unstructured":"Johnson CS, , Holzemer NF, , Wingert RA. Laser ablation of the zebrafish pronephros to study renal epithelial regeneration. J Vis Exp, 2011; 54:pii: 2845.","journal-title":"J Vis Exp"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.04.012"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3081"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2013.06.063"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.08.052"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077857"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio732"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_3_3_21_2","volume-title":"The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio rerio)","author":"Westerfield M","year":"2000","unstructured":"Westerfield M: The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio rerio). University of Oregon Press, Eugene, 2000."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.3652892"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00844"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(99)70088-0"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060109"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00870.2002"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1195929"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047644"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044231"},{"key":"e_1_3_3_30_2","first-page":"S39","article-title":"Review of mitral valve insufficiency: repair or replacement","volume":"6","author":"Madesis A","year":"2014","unstructured":"Madesis A, , Tsakiridis K, , Zarogoulidis P, et al. Review of mitral valve insufficiency: repair or replacement. J Thorac Dis, 2014; 6:S39\u2013S51.","journal-title":"J Thorac Dis"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11045"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(04)00133-9"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09249"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.2007.00959.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.98"}],"container-title":["Zebrafish"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/zeb.2014.1027","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/zeb.2014.1027","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/zeb.2014.1027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T15:13:58Z","timestamp":1773242038000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/zeb.2014.1027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,1]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,12,1]]}},"alternative-id":["10.1089\/zeb.2014.1027"],"URL":"https:\/\/doi.org\/10.1089\/zeb.2014.1027","relation":{},"ISSN":["1545-8547","1557-8542"],"issn-type":[{"value":"1545-8547","type":"print"},{"value":"1557-8542","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,1]]}}}