{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:27:15Z","timestamp":1792466835383,"version":"4.5.0"},"reference-count":37,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T00:00:00Z","timestamp":1466553600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Zebrafish"],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Thrombosis is a leading cause of death and the development of effective and safe therapeutic agents for thrombotic diseases has been proven challenging. In this study, taking advantage of the transparency of larval zebrafish, we developed a larval zebrafish thrombosis model for drug screening and efficacy assessment. Zebrafish at 2\u2009dpf (days post fertilization) were treated with phenylhydrazine (PHZ) and a testing drug for 24\u2009h. Tested drugs were administered into the zebrafish either by direct soaking or circulation microinjection. Antithrombotic efficacy was quantitatively evaluated based on our previously patented technology characterized as an image analysis of the heart red blood cells stained with O-dianisidine staining. Zebrafish at 2\u2009dpf treated with PHZ at a concentration of 1.5\u2009\u03bcM for a time period of 24\u2009h were determined as the optimum conditions for the zebrafish thrombosis model development. Induced thrombosis in zebrafish was visually confirmed under a dissecting stereomicroscope and quantified by the image assay. All 6 human antithrombotic drugs (aspirin, clopidogrel, diltiazem hydrochloride injection, xuanshuantong injection, salvianolate injection, and astragalus injection) showed significant preventive and therapeutic effects on zebrafish thrombosis (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05,\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01, &amp;\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.001) in this zebrafish thrombosis model. The larval zebrafish thrombosis model developed and validated in this study could be used for\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    thrombosis studies and for rapid screening and efficacy assessment of antithrombotic drugs.\n                  <\/jats:p>","DOI":"10.1089\/zeb.2016.1263","type":"journal-article","created":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T22:03:44Z","timestamp":1466719424000},"page":"335-344","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":88,"title":["A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs"],"prefix":"10.1177","volume":"13","author":[{"given":"Xiao-Yu","family":"Zhu","sequence":"first","affiliation":[{"name":"Wenzhou Medical University","place":["People's Republic of China"]},{"name":"Hunter Biotechnology, Inc.","place":["People's Republic of China"]},{"name":"Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Cui","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunter Biotechnology, Inc.","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Ya","family":"Guo","sequence":"additional","affiliation":[{"name":"Hunter Biotechnology, Inc.","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Xia","sequence":"additional","affiliation":[{"name":"Hunter Biotechnology, Inc.","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru-Shun","family":"Song","sequence":"additional","affiliation":[{"name":"Hunter Biotechnology, Inc.","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao-Cong","family":"Lao","sequence":"additional","affiliation":[{"name":"Hunter Biotechnology, Inc.","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao-Xian","family":"Xuan","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals","place":["People's Republic of China"]},{"name":"Center of Safety Evaluation","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Qi","family":"Li","sequence":"additional","affiliation":[{"name":"Wenzhou Medical University","place":["People's Republic of China"]},{"name":"Hunter Biotechnology, Inc.","place":["People's Republic of China"]},{"name":"Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,6,22]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2174\/138161212799504731"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21334"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2004.00999.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000075"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/bcmd.2002.0568"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.03815.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/857058"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131109398"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01763.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111958"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2012.01.004"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2014.11.011"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199105000-00001"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767040-00013"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3565(25)22150-4"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.04.048"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-011-0690-6"},{"key":"e_1_3_3_19_2","first-page":"247948","article-title":"Cardiovascular effects of salvianolic acid b","volume":"2013","author":"Wang J","year":"2013","unstructured":"Wang J, , Xiong X, , Feng B. 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