{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:41:22Z","timestamp":1773308482281,"version":"3.50.1"},"reference-count":29,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Zebrafish"],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In 2017, the zebrafish unit at University of Glasgow experienced a detrimental outbreak of pathogenic bacterium,\n                    <jats:italic toggle=\"yes\">Mycobacterium haemophilum<\/jats:italic>\n                    . The presence of other bacterial species was also confirmed by bacteriology growth in the same unit. The affected individuals composed of a wild-origin parental population sourced from India and their F1 offspring generation. Bacteria were diagnostically confirmed to be present systemically in fish and within the water and biofilm of the recirculating zebrafish system. In the absence of a publicly accessible step-by-step disinfectant protocol for these difficult-to-eliminate pathogens, we devised a successful procedure to eradicate mycobacteria and\n                    <jats:italic toggle=\"yes\">Aeromonas<\/jats:italic>\n                    species after colony removal using Cleanline Chlorine tablets (active ingredient Sodium dichloroisocyanurate) and Virkon Aquatic\n                    <jats:sup>\u00ae<\/jats:sup>\n                    . Postdisinfection diagnostics did not detect pathogens in the system or in the new fish inhabiting the system that were tested. Newly established fish colonies have not shown similar clinical signs or disease-induced mortality in the 1-year period following system disinfection and repopulation. We present a historical background of the bacterial outbreak and a disinfection method which can be replicated in other zebrafish facilities\u2014at small or large scales\u2014for reliable mycobacterium removal. This procedure can be implemented as a disinfection protocol before the introduction of a new fish population to a previously contaminated system.\n                  <\/jats:p>","DOI":"10.1089\/zeb.2018.1628","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T13:48:26Z","timestamp":1540475306000},"page":"77-86","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Overview of a Disease Outbreak and Introduction of a Step-by-Step Protocol for the Eradication of\n                    <i>Mycobacterium haemophilum<\/i>\n                    in a Zebrafish System"],"prefix":"10.1177","volume":"16","author":[{"given":"Anita","family":"R\u00e1cz","sequence":"first","affiliation":[{"name":"Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toni","family":"Dwyer","sequence":"additional","affiliation":[{"name":"Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom."},{"name":"Institute of Aquaculture, University of Stirling, Stirling, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaun S.","family":"Killen","sequence":"additional","affiliation":[{"name":"Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,10,24]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.13278"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2007.04.077"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2012.0819"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","unstructured":"N\u00fcsslein-Volhard C Dahm R: Zebrafish\u2014A Practical Approach. 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