{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T23:59:20Z","timestamp":1780617560184,"version":"3.54.1"},"reference-count":43,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:00:00Z","timestamp":1559174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Zebrafish"],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Envenomation by the Venezuelan bushmaster snake (\n                    <jats:italic toggle=\"yes\">Lachesis muta muta<\/jats:italic>\n                    ) (Serpentes: Viperidae) is characterized by local and cardiac alterations. This study investigates the\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    cardiac dysfunction, tissue destruction, and cellular processes triggered by\n                    <jats:italic toggle=\"yes\">Lachesis muta muta<\/jats:italic>\n                    snake crude venom and a C-type lectin (CTL)-like toxin named Mutacytin-1 (MC-1). The 28\u2009kDa MC-1 was obtained by molecular exclusion, ion exchange, and C-18 (checking pureness) reverse-phase chromatographies. N-terminal sequencing of the first eight amino acids (NNCPQ LLM) revealed 100% identity with Mutina (CTL-like) isolated from\n                    <jats:italic toggle=\"yes\">Lachesis stenophrys<\/jats:italic>\n                    , which is a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -dependent-type galactoside-binding lectin from\n                    <jats:italic toggle=\"yes\">Bothrops jararaca<\/jats:italic>\n                    and CTL BpLec from\n                    <jats:italic toggle=\"yes\">Bothrops pauloensis.<\/jats:italic>\n                    The cardiotoxicity in zebrafish of MC-1 was evaluated by means of specific phenotypic expressions and larvae behavior at 5, 15, 30, 40 and 60\u2009min post-treatment. The\n                    <jats:italic toggle=\"yes\">L. muta muta<\/jats:italic>\n                    venom and MC-1 also produced heart rate\/rhythm alterations, circulation modifications, and the presence of thrombus and apoptotic phenomenon with pericardial damages. Acridine orange (100\u2009\u03bcg\/mL) was used to visualize apoptosis cellular process in control and treated whole embryos. The cardiotoxic alterations happened in more than 90% of all larvae under the action of\n                    <jats:italic toggle=\"yes\">L. muta muta<\/jats:italic>\n                    venom and MC-1. The findings have demonstrated the potential cardiotoxicity by\n                    <jats:italic toggle=\"yes\">L. muta muta<\/jats:italic>\n                    venom, suggesting the possibility of cardiovascular damages to patients after bushmaster envenoming.\n                  <\/jats:p>","DOI":"10.1089\/zeb.2019.1731","type":"journal-article","created":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T11:11:47Z","timestamp":1559214707000},"page":"379-387","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Mutacytin-1, a New C-Type Lectin-Like Protein from the Venezuelan Cuaima (\n                    <i>Lachesis muta muta<\/i>\n                    Linnaeus, 1766) (Serpentes: Viperidae) Snake Venom Inducing Cardiotoxicity in Developing Zebrafish (\n                    <i>Danio rerio<\/i>\n                    ) Embryos"],"prefix":"10.1177","volume":"16","author":[{"given":"Yurisbeth","family":"Zanotty","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Ultraestructura, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Universidad Central de Venezuela, Caracas, Venezuela."},{"name":"Secci\u00f3n de Microscopia Electr\u00f3nica, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Microscopia Electr\u00f3nica, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Perdomo","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Microscopia Electr\u00f3nica, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elda E.","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Natural Toxins Research Center, Texas A&amp;M University-Kingsville, Kingsville, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda E.","family":"Giron","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Ultraestructura, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montamas","family":"Suntravat","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Natural Toxins Research Center, Texas A&amp;M University-Kingsville, Kingsville, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belsy","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiopatolog\u00eda, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Ibarra","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiopatolog\u00eda, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyibeth","family":"Montero","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Ultraestructura, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Medina","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Ultraestructura, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis F.","family":"Navarrete","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Ultraestructura, Instituto Anat\u00f3mico \u201cJos\u00e9 Izquierdo,\u201d Universidad Central de Venezuela, Caracas, 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